Functional Genetic Variant in ATG5 Gene Promoter in Acute Myocardial Infarction
Table 1
PCR primers for the human ATG5 gene promoter.
PCR primers
DNA sequences
Location
Position
Products
Sequencing
ATG5-F1
5′-GGCATGCTTCCCTAACTTGA-3′
106326889
−1069 bp
649 bp
ATG5-R1
5′-CCCACCCATCCAAGAGTACA-3′
106326241
−421 bp
ATG5-F2
5′-TCTCGATCTCCTGACCTCGT-3′
106326375
−655 bp
658 bp
ATG5-R2
5′-CACTTCCGCCCTCTGGTAT-3′
106325718
+103 bp
Functioning
ATG5-F
5′-(KpnI)-GGCATGCTTCCCTAACTTGA-3′
106326889
−1069 bp
1172 bp
ATG5-R
5′-(HindIII)-CACTTCCGCCCTCTGGTAT-3′
106325718
+103 bp
PCR primers are designed based on the genomic DNA sequence of the ATG5 gene (NC_000006.12). The transcription start site is at the position of 106325820 (+1).