Functional Genetic Variant in ATG5 Gene Promoter in Acute Myocardial Infarction

Table 1

PCR primers for the human ATG5 gene promoter.

PCR primers	DNA sequences	Location	Position	Products
Sequencing
ATG5-F1	5′-GGCATGCTTCCCTAACTTGA-3′	106326889	−1069 bp	649 bp
ATG5-R1	5′-CCCACCCATCCAAGAGTACA-3′	106326241	−421 bp
ATG5-F2	5′-TCTCGATCTCCTGACCTCGT-3′	106326375	−655 bp	658 bp
ATG5-R2	5′-CACTTCCGCCCTCTGGTAT-3′	106325718	+103 bp
Functioning
ATG5-F	5′-(KpnI)-GGCATGCTTCCCTAACTTGA-3′	106326889	−1069 bp	1172 bp
ATG5-R	5′-(HindIII)-CACTTCCGCCCTCTGGTAT-3′	106325718	+103 bp

PCR primers are designed based on the genomic DNA sequence of the ATG5 gene (NC_000006.12). The transcription start site is at the position of 106325820 (+1).