miR-149 Alleviates Ox-LDL-Induced Endothelial Cell Injury by Promoting Autophagy through Akt/mTOR Pathway

<div>Effect of miR-149 mimics on autophagy in ox-LDL-induced HUVECs. (a) HUVECs of control, ox-LDL, and miR-149 mimics were stained with LC3-II antibody (green, magnification ×100). The nucleus was stained with DAPI (blue). (b) Quantification of LC3-II fluorescence intensity, as presented by cells with LC3-II dots. miR-149 mimics significantly increase the number of cells with LC3-II dots. (c) Western blot was performed to determine LC3-I, LC3-II, and P62 proteins. (d) miR-149 mimics significantly increases the ratio of LC3-II to LC3-I. 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d="M384 0V27C293 34 287 42 287 114V635C232 613 172 594 109 583V559L157 557C201 555 205 550 205 499V114C205 42 199 34 109 27V0H384Z"></path></g></svg> vs. ox-LDL group.</div>

Cardiology Research and Practice

fig2

Figure 2

Figure 2: miR-149 Alleviates Ox-LDL-Induced Endothelial Cell Injury by Promoting Autophagy through Akt/mTOR Pathway 