TMAO-Activated Hepatocyte-Derived Exosomes Are Widely Distributed in Mice with Different Patterns and Promote Vascular Inflammation

Table 1

qPCR primers for mRNA (Mus musculus) used in the study.

Name	Forward sequence	Reverse sequence
Phgdh	CCAGGTGGTTACACAAGGAACA	TTCACGTCTGCCTGCTTAGATG
Mdh2	TTTGTGGCAGAGCTAAAGGGTT	GTACACTGAGAGATCAGGGGGA
Echs1	AATGGAGATGGTCCTCACTGGT	TCTGCACATTGGATGGCTTCTT
Rap2a	CAGCAGAGCTTCCAAGACATCA	CTCTCCAGGTCCACTTTGTTCC
Gpd1l	TCCGACATCATCCGAGAGAAGA	AAGGCCGTTCTGCATCACTTT
Slc3a2	GGCCCAATTCACAAGAACCAGA	TGGGAGTGAGGTCCAAAATGATG
Hyou1	GATCTTCGGGTATTTGGCTCCC	TAAAGTGGGCCTTGATGCCTTT
Hspa9	AACTCCTGTGTGGCTGTTATGG	CAAGTCGTTCTCCATCTGCTGT
Psat1	GGGTGGAGTTTGACTTCGTACC	TCTTCTGAGCACCAGCGAAAAT
Pck2	GAGGCTGAGAACACTGCCATAC	TGCGAAGGAGTTACAATCACCG
Tnf	CTGTAGCCCACGTCGTAGC	TTGAGATCCATGCCGTTG
Icam1	CCCACGCTACCTCTGCTC	GATGGATACCTGAGCATCACC
Sele	ATGCCTCGCGCTTTCTCTC	GTAGTCCCGCTGACAGTATGC
Cox-2	TTCAACACACTCTATCACTGGC	AGAAGCGTTTGCGGTACTCAT
Gapdh	ACTCTTCCACCTTCGATGCC	TGGGATAGGGCCTCTCTTGC

Phgdh: 3-phosphoglycerate dehydrogenase; Mdh2: malate dehydrogenase 2, NAD (mitochondrial); Echs1: enoyl coenzyme A hydratase, short chain, 1, mitochondrial; Rap2a: Ras-related protein 2a; Gpd1l: glycerol-3-phosphate dehydrogenase 1-like; Slc3a2: solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2; Hyou1: hypoxia upregulated 1; Hspa9: heat shock protein 9; Psat1: phosphoserine aminotransferase 1; Pck2: phosphoenolpyruvate carboxykinase 2 (mitochondrial); Tnf: tumor necrosis factor; Icam1: intercellular adhesion molecule-1; Sele: selectin, endothelial cell; Cox-2: cyclooxygenase-2; Gapdh glyceraldehyde-3-phosphate dehydrogenase.