miR-197 Participates in Lipopolysaccharide-Induced Cardiomyocyte Injury by Modulating SIRT1

Figure 3

Effects of miR-197 inhibition on the expressions of SIRT1 and p35 in LPS-induced H9C2 cells. (a, b) Western blot was performed to measure the protein level of SIRT1 in LPS-induced H9C2 cells transfected with or without the miR-197 inhibitor. GAPDH was used as the loading control. (c) The mRNA expression of SIRT1 was then determined by qRT-PCR. GAPDH was used as the loading control. (d, e) The protein levels of p53 and AC-p53 in LPS-induced H9C2 cells transfected with or without the miR-197 inhibitor were evaluated via western blot. GAPDH was used as the loading control. vs. control; vs. LPS + IC. SIRT1, silent information regulator 1; LPS, lipopolysaccharide; qRT-PCR, quantitative real-time polymerase chain reaction; AC-p53, acetyl-p53; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; I, miR-197 inhibitor; IC, inhibitor control.

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