Analysis of a Family with Brugada Syndrome and Sudden Cardiac Death Caused by a Novel Mutation of SCN5A

Table 1

Design of reverse transcription primers for qPCR detection of SCN5A in peripheral blood.

Primer name	Primer sequence
SCN5A mutation site pre-qPCR F	5′-CCAGACAGAGGGAGACTTGC-3′
SCN5A mutation site pre-qPCR R	3′-CTGGAGTCCACAGCTGCATA-5′
qPCR after SCN5A mutation site F	5′-CGCCTACGTGATGAGTGAGA-3′
qPCR after SCN5A mutation site R	3′-GTCGGCGAGATCTTCACTGT-5′
hGAPDH F	5′-CAAGGTCATCCATGACAACTTTG-3′
hGAPDH R	3′-GTCCACCACCCTGTTGCTGTAG-5′

F as forward and R as reverse.