Sean Clark, Michael A. Mcguckin, Terry Hurst, Bruce G. Ward, "Effect Of Interferon-γ and TNF-α on MUCl MUCIN Expression in Ovarian Carcinoma Cell Lines", Disease Markers, vol. 12, Article ID 480236, 8 pages, 1994. https://doi.org/10.1155/1994/480236
Effect Of Interferon-γ and TNF-α on MUCl MUCIN Expression in Ovarian Carcinoma Cell Lines
In view of the potential uses of cell surface tumour associated antigens in novel anticancer treatment. a study was designed to investigate whether the biological response modifiers interferon-gamma (IFN-γ) and tumour necrosis factor-alpha (TNF-α) could effect the expression of an epitope on the tumour associated MUC I epithelial mucin. Four ovarian carcinoma cell lines showing high (OAW42 and GG) and low (JAM and PEO1) basal expression of MUC1 were treated with 10-1000 U/mL of IFN-γor TNF-α for one or five days. Changes in MUC1 expression in cells exposed to IFN-γ or TNF-α were monitored using an ELISA technique with the monoclonal antibody BC2 which reacts with a core protein epitope on the MUC1 mucin, and then corrected for the number of viable cells present. TNF-α had little effect on MUC1 expression, but one or five days exposure to IFN-γ significantly increased MUC1 expression (p < 0.01) in all cell lines including the two cell lines that initially showed little or no expression.
Copyright © 1994 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.