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Disease Markers
Volume 12 (1994), Issue 3, Pages 187-197
http://dx.doi.org/10.1155/1994/603420

Production and Characterisation of Anti-Cardiac Troponin-I Monoclonal Antibodies

Kh. H. Haider1 and W. H. Stimson2

1Faculty of Pharmacy, University of the Punjab, Lahore, Pakistan
2Department of Immunology, University of Strathclyde, The Todd Centre, Glasgow, UK

Received 13 May 1994

Copyright © 1994 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Cardiac troponin-I (cTn-I) was isolated from bovine left ventricular tissue and used as immunogen. Sixteen murine hybridoma lines were produced with two of them. I D 12 and 5F4, showing a high specificity for cTn-I; both of these monoclonal antibodies (McAbs) were isotyped as IgG I with kappa - light chains. The specificity of the McAbs for cTn-1 was confirmed by ELISA, western blotting and by the ability of the antibodies to block actomyosin ATPase inhibition by cTn-I. The McAbs may be useful for human ill vivo imaging of myocardial infarcts and other pathological conditions related to cardiac myocyte damage.