Disease Markers

Disease Markers / 2000 / Article

Open Access

Volume 16 |Article ID 953059 | https://doi.org/10.1155/2000/953059

S. Feki, H. El Omri, M. A. Laatiri, S. Ennabli, K. Boukef, F. Jenhani, "Contribution of Flow Cytometry to Acute Leukemia Classification in Tunisia", Disease Markers, vol. 16, Article ID 953059, 3 pages, 2000. https://doi.org/10.1155/2000/953059

Contribution of Flow Cytometry to Acute Leukemia Classification in Tunisia

Received14 Apr 2000
Accepted11 May 2000


The precision of immunological characterization of leukemias was improved by a certain number of technical innovations, particularly hybridoma production and standardization, resulting in monoclonal antibodies and definition of recognised cellular antigens (designated by CD: Cluster of Differentiation).The aim of this work was to determine the immunophenotyping profile of patients with leukemia, by means of a flow cytometric method: 66 blood samples coming from leukemic persons in the Sahel region were studied by flow cytometry, using about thirty monoclonal antibodies all marked with a fluorochrome, in one or two colour systems to assess their distribution according to type (lymphoid B or T / myeloid) and age, and to search for possible co-expressions of markers of different lineages.The marked preponderance of childhood B-ALL in our series is, at least partly, attributable to the age distribution of the Tunisian population. In agreement with studies from other countries, the majority of AML cases occurred among adults. A high proportion of AML cases in our series co-expressed markers of other lineages. Overall, accurate classification of acute leukemias was possible from a simple peripheral blood sample in 62 of 66 cases (93.9%).

Copyright © 2000 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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