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Disease Markers
Volume 21, Issue 2, Pages 75-77
Short Report

Two Polymorphisms in the Epithelial Cell-Derived Neutrophil-Activating Peptide (ENA-78) Gene

Mahsa M. Amoli,1 Bagher Larijani,1 Wendy Thomson,2 William E. R. Ollier,2 and Miguel A. Gonzalez-Gay3

1Endocrinology and Metabolism Research Centre, Tehran University of Medical Sciences, Tehran, Iran
2Centre for Integrated Genomic Medical Research, Manchester University Medical School, Manchester, UK, USA
3Divisions of Rheumatology and Pediatrics Hospital Xeral-Calde, Lugo, Spain

Received 24 May 2005; Accepted 24 May 2005

Copyright © 2005 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Increased expression of epithelial cell-derived neutrophil-activating peptide (ENA-78) has been reported in several immune and inflammatory conditions suggesting its role in inflammatory response. We have identified two single nucleotide polymorphisms in the promoter and exon 2 of the ENA-78 gene by scanning the full length gene using DHPLC DNA fragment analysis and DNA sequencing.

The polymorphism at position +398 (A/G from the first ATG codon) in exon 2 results in a synonymous substitution not resulting in an amino acid change. The promoter polymorphism was found at position −156 (C/G from the first ATG codon). An assay was designed for the detection of the polymorphisms using SNapshot ddNTP primer extension, followed by capillary electrophoresis (ABI 3100).

Allele and genotype frequencies for the promoter −156 polymorphism are presented for 107 healthy Spanish and 54 UK Caucasians. Frequencies for the exon 2 polymorphism are also presented for 63 UK Caucasians.