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Disease Markers
Volume 22, Issue 3, Pages 119-125
http://dx.doi.org/10.1155/2006/347502

Altered β-Amyloid Precursor Protein Isoforms in Mexican Alzheimer’s Disease Patients

V. J. Sánchez-González,1,2 G. G. Ortiz,1 P. Gallegos-Arreola,2 M. A. Macías-Islas,3 E. D. Arias-Merino,4 V. Loera-Castañeda,1 E. Martínez-Cano,1 I. E. Velázquez-Brizuela,1 S. A. Rosales-Corral,1 C. R. Curiel-Ortega,3 F. Pacheco-Moisés,5 and J. J. García6

1Laboratorio de Desarrollo-Envejecimiento, Enfermedades Neurodegenerativas, División de Neurociencias, Centro de Investigación Biomédica de Occidente (CIBO), Instituto Mexicano del Seguro Social (IMSS), Guadalajara, Jalisco, Mexico
2Laboratorio de Biología Molecular, División de Genética, CIBO-IMSS, Guadalajara, Jalisco, Mexico
3Departamento de Neurología, Hospital de Especialidades del Centro Médico Nacional de Occidente, IMSS, Guadalajara, Jalisco, México, and Departamento de Neurociencias CUCS, Universidad de Guadalajara, Mexico
4Departamento de Salud Pública, Centro Universitario de Ciencias de la Salud, U de G. Guadalajara, Jalisco, Mexico
5Departamento de Química, Centro Universitario de Ciencias Exactas e Ingeniería, U de G. Guadalajara, Jalisco, Mexico
6Departamento de Bioquímica, Instituto Nacional de Cardiología, Mexico

Received 29 May 2006; Accepted 29 May 2006

Copyright © 2006 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective: To determine the β-amyloid precursor protein (βAPP) isoforms ratio as a risk factor for Alzheimer’s Disease and to assess its relationship with demographic and genetic variables of the disease.

Methods: Blood samples from 26 patients fulfilling NINCDS-ADRDA diagnostic criteria for AD and 46 healthy control subjects were collected for Western blotting for βAPP. A ratio of βAPP isoforms, in optical densities, between the upper band (130 Kd) and the lower bands (106–110 Kd) was obtained. Odds ratios were obtained to determine risk factor of this component.

Results: βAPP ratio on AD subjects was lower than that of control subjects: 0.3662 ± 0.1891 vs. 0.6769 ± 0.1021 (mean ± SD, p<0.05). A low βAPP ratio (<0.6) showed an OR of 4.63 (95% CI 1.45 ± 15.33). When onset of disease was taken into account, a βAPP ratio on EOAD subjects of 0.3965 ± 0.1916 was found vs. 0.3445 ± 0.1965 on LOAD subjects (p>0.05).

Conclusions: Altered βAPP isoforms is a high risk factor for Alzheimer’s disease, although it has no influence on the time of onset of the disease.