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Disease Markers
Volume 26, Issue 2, Pages 49-53

Variability of Thiobarbituric Acid Reacting Substances in Saliva

Michal Behuliak,1,2 Roland Pálffy,1,2 Roman Gardlík,1,2 Július Hodosy,1,3 Lukáč Halčák,4 and Peter Celec1,2,5

1BiomeD Research & Publishing Group, USA
2Institute of Pathophysiology, Faculty of Medicine, Comenius University, Bratislava, Slovakia
3Institute of Physiology, Faculty of Medicine, Comenius University, Bratislava, Slovakia
4Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine, Comenius University, Bratislava, Slovakia
5Department of Molecular Biology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia

Received 21 April 2009; Accepted 21 April 2009

Copyright © 2009 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Introduction: Salivary TBARS are a potential marker of oxidative stress in the oral cavity. Previous studies have found increased levels of salivary TBARS in various diseases. The aim of this study was to assess the variability of salivary TBARS in both genders.

Subjects & Methods: Saliva samples from thirty-eight healthy volunteers (18F & 20M) were collected every day during 30 day period. TBARS levels were measured spectrophotometrically using a high-throughput 96-well plate method. Time series analysis was performed using standard statistical methods.

Results: Repeated measures ANOVA showed a significant variation of salivary TBARS within day and subjects (p < 0.001). The dynamics did not differ between genders. Intraindividual variability was very high in both genders with coefficients of variation of more than 60%. Interindividual variability was higher in men than in women (73% vs. 46%; p < 0.01).

Discussion: The relatively high intraindividual variability indicates that the use of salivary TBARS will be limited to research on a population level, although some informative value might be gained by repeated samplings. Factors influencing the biological variability of salivary TBARS should be identified in further studies.