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Disease Markers
Volume 29 (2010), Issue 1, Pages 21-29
http://dx.doi.org/10.3233/DMA-2010-0722

14-3-3 Sigma And p53 Expression in Gastric Cancer and Its Clinical Applications

Gilbert Mühlmann,1 Dietmar Öfner,2 Matthias Zitt,1 Hannes M. Müller,1 Hans Maier,3 Patrizia Moser,3 Kurt W. Schmid,4 Marion Zitt,1 and Albert Amberger5

1Center of Operative Medicine, Department of Visceral, Transplant and Thoracic Surgery, Innsbruck Medical University, Austria
2Department of Surgery, Paracelsus Private Medical University, Salzburger Landeskliniken, Salzburg, Austria
3Department of Pathology, Innsbruck Medical University, Austria
4Institute of Pathology and Neuropathology, University Hospital Essen, Germany
5Tyrolean Cancer Research Institute, Innsbruck, Austria

Received 2 September 2010; Accepted 2 September 2010

Copyright © 2010 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

14-3-3 sigma (σ) induces G2 arrest enabling the repair of damaged DNA. The function of 14-3-3 σ is frequently lost in tumor cells, indicating a potential tumor suppressor function. The purpose of this study was to evaluate the prognostic value of 14-3-3 σ expression in human gastric cancer. 14-3-3 σ expression was analyzed by immunohistochemistry in 157 tumor samples of patients, who underwent resection for gastric cancer. Since 14-3-3 σ is involved in the p53 network, p53 expression was detected in parallel and correlated with 14-3-3 σ. 14-3-3 σ was found to be overexpressed in 75 (47.8%) of 157 cases, the overexpression rate of p53 protein was 27.4%. 14-3-3 σ overexpression was statistically significantly associated with pT-stage (p=0.041) pN-stage (p=0.015) and UICC-stage (p=0.019) and showed a borderline significance with Lauren classification (p=0.057). Univariate survival calculations revealed a coexistent 14-3-3 σ and p53 overexpression as a significant predictor of disease-free survival. Multivariate analysis did not unfold 14-3-3 as an independent prognostic factor for disease-free survival and overall survival. Concomitant 14-3-3 σ and p53 overexpression in tumor cells of patients with gastric cancer identifies a population of patients with relatively unfavorable prognosis.