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Disease Markers
Volume 31, Issue 1, Pages 25-32
http://dx.doi.org/10.3233/DMA-2011-0797

Gene Expression Changes in Femoral Head Necrosis of Human Bone Tissue

Bernadett Balla,1 Csaba Pintér,2 János P. Kósa,1 János Podani,3 István Takács,1 Zsolt Nagy,1 Gábor Speer,1 Balázs Horváth,2 László Korányi,4 and Péter Lakatos1

11st Department of Internal Medicine, Semmelweis University, Budapest, Hungary
2Department of Traumatology and Orthopaedics, Csolnoky Ferenc Veszprém County Hospital, Veszprém, Hungary
3Department of Plant Taxonomy and Ecology, Eötvös Loránd University, Budapest, Hungary
4Drug Research Center, Balatonfüred, Hungary

Received 21 July 2011; Accepted 21 July 2011

Copyright © 2011 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Osteonecrosis of the femoral head (ONFH) is the result of an interruption of the local circulation and the injury of vascular supply of bone. Multiple factors have been implicated in the development of the disease. However the mechanism of ischemia and necrosis in non-traumatic ONFH is not clear. The aim of our investigation was to identify genes that are differently expressed in ONFH vs. non-ONFH human bone and to describe the relationships between these genes using multivariate data analysis. Six bone tissue samples from ONFH male patients and 8 bone tissue samples from non-ONFH men were examined. The expression differences of selected 117 genes were analyzed by TaqMan probe-based quantitative real-time RT-PCR system. The significance test indicated marked differences in the expression of nine genes between ONFH and non-ONFH individuals. These altered genes code for collagen molecules, an extracellular matrix digesting metalloproteinase, a transcription factor, an adhesion molecule, and a growth factor. Canonical variates analysis demonstrated that ONFH and non-ONFH bone tissues can be distinguished by the multiple expression profile analysis of numerous genes controlled via canonical TGFB pathway as well as genes coding for extracellular matrix composing collagen type molecules. The markedly altered gene expression profile observed in the ONFH of human bone tissue may provide further insight into the pathogenetic process of osteonecrotic degeneration of bone.