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Disease Markers
Volume 31, Issue 4, Pages 211-214
http://dx.doi.org/10.3233/DMA-2011-0818

Absence of kl-vs Variant of Klotho Gene in Iranian Cardiac Patients (Comparison to the World Populations)

Javad Tavakkoly-Bazzaz,1,2 Ozra Tabatabaei-Malazy,1 Mohammad Tajmir-Riahi,2 Daryoosh Javidi,2 Manizhe Izadi,1 Maryam Shahrabi-Farahani,1 Parvin Amiri,1 and Mahsa M. Amoli1

1Endocrinology and Metabolism Research Center, Tehran University of Medical Sciences, Tehran, Iran
2Department of Medical Genetics, Tehran University of Medical Sciences, Tehran, Iran

Received 14 October 2011; Accepted 14 October 2011

Copyright © 2011 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective: Klotho has an important role in development of coronary artery (CAD) disease. A functional variant of klotho gene (kl-vs) has been found as an independent risk factor for early-onset occult coronary artery disease (CAD) in previous studies. The Frequency of this variant was not known in Iranian population. We have examined the allele frequency of the kl-vs variant in a case-control study in an Iranian population.

Methods and results: Genotyping for kl-vs variant was carried out in N = 107 individuals including N = 54 cases and N = 53 control who all underwent coronary angiogram for CAD evaluation. Patients with >50% stenosis in vessels considered as case groups (or CAD+) and patients with normal vessels (or CAD) as controls. The frequency of kl-vs variant was determined in these patients using PCR-RFLP technique. None of the individual was carrying the kl-vs mutation in our samples. The frequency of kl-vs mutation was significantly different from previous studies in different populations.

Conclusion: The kl-vs variant seems to be scare found in the Iranian population in comparison to other populations reported previously. Klotho gene might be a candidate gene of atherosclerosis in some populations but not in Iranian population. Further studies are required to examine the frequency of kl-vs variant in other populations from the Middle East.