Table of Contents Author Guidelines Submit a Manuscript
Disease Markers
Volume 31, Issue 4, Pages 241-246
http://dx.doi.org/10.3233/DMA-2011-0823

A Polymorphism Within the Connective Tissue Growth Factor (CTGF) Gene has No Effect on Non-Invasive Markers of Beta-Cell Area and Risk of Type 2 Diabetes

Olga Pivovarova,1,2 Eva Fisher,3 Katarzyna Dudziak,4 Iryna Ilkavets,5 Steven Dooley,5 Petr Slominsky,6 Svetlana Limborska,6 Martin O. Weickert,1,2,7 Joachim Spranger,1,2 Andreas Fritsche,4 Heiner Boeing,3 Andreas F. H. Pfeiffer,1,2 and Natalia Rudovich1,2

1Department of Clinical Nutrition, German Institute of Human Nutrition, Potsdam-Rehbruecke, Nuthetal, Germany
2Department Endocrinology, Diabetes and Nutrition, Charité University, Medicine, Campus Benjamin Franklin, Berlin, Germany
3Department of Epidemiology, German Institute of Human Nutrition, Potsdam-Rehbruecke, Nuthetal, Germany
4Division of Endocrinology, Diabetology, Angiology, Nephrology, and Clinical Chemistry, Department of Internal Medicine, University Hospital, Tübingen and Eberhard Karls University Tübingen, Member of the German, Center for Diabetes Research (DZD), Tübingen, Germany
5Molecular Hepatology – Alcohol Associated Diseases, Medical Clinic II, Faculty of Medicine Mannheim at Heidelberg University, Mannheim, Germany
6Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia
7Warwickshire Institute for the Study of Diabetes, Endocrinology and Metabolism, University Hospitals Coventry and Warwickshire, Coventry, UK and Clinical Sciences Research Institute, Warwick Medical School, University of Warwick, Coventry, UK

Received 14 October 2011; Accepted 14 October 2011

Copyright © 2011 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Chromosomal locus 6q23 is strongly linked to type 2 diabetes (T2DM) and related features including insulin secretion in various ethnic populations. Connective tissue growth factor (CTGF) gene is an interesting T2DM candidate gene in this chromosome region. CTGF is a key mediator of progressive pancreatic fibrosis up-regulated in type 2 diabetes. In contrast, CTGF inactivation in mice compromises islet cell proliferation during embryogenesis. The aim of our study was to investigate an impact of CTGF genetic variation on pancreatic beta-cell function and T2DM pathogenesis. We studied the effect of a common CTGF polymorphism rs9493150 on the risk of the T2DM development in three independent German cohorts. Specifically, the association between CTGF polymorphism and non-invasive markers of beta-cell area derived from oral glucose tolerance test was studied in subjects without diabetes. Neither in the Metabolic Syndrome Berlin Potsdam (MESYBEPO) study (n = 1026) (OR = 0.637, CI (0.387–1.050); p = 0.077) nor in the European Prospective Investigation into Cancer and Nutrition-Potsdam (EPIC-Potsdam) (n = 3049) cohort (RR = 0.77 CI (0.49–1.20), p = 0.249 for the recessive homozygote in general model), a significant association with increased diabetes risk was observed. The risk allele of rs9493150 had also no effect on markers of beta-cell area in the combined analysis of the MESYBEPO and Tübingen Family Study (n = 1826). In conclusion, the polymorphism rs9493150 in the 5’-untranslated region of the CTGF gene has no association with T2DM risk and surrogate markers of beta-cell area.