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Disease Markers
Volume 32, Issue 4, Pages 273-279
http://dx.doi.org/10.3233/DMA-2011-0881

E3B1/ABI-1 Isoforms Are Down-Regulated in Cancers of Human Gastrointestinal Tract

Rafia A. Baba,1 Hina F. Bhat,1 Lateef A. Wani,2 Muneesa Bashir,1 Mudasir M. Wani,3 Sumyra K. Qadri,4 and Firdous A. Khanday1

1Department of Biotechnology, University of Kashmir, Srinagar, Jammu and Kashmir, India
2Department of Pathology, Shri Maharaja Hari Singh Hospital, Srinagar, Jammu and Kashmir, India
3Department of Surgery, Shri Maharaja Hari Singh Hospital, Srinagar, Jammu and Kashmir, India
4Department of Pathology, Sher-i-Kashmir Institute of Medical Sciences, Soura, Srinagar, Jammu and Kashmir, India

Received 15 March 2012; Accepted 15 March 2012

Copyright © 2012 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The expression of E3B1/ABI-1 protein and its role in cancer progression and prognosis are largely unknown in the majority of solid tumors. In this study, we examined the expression pattern of E3B1/ABI-1 protein in histologically confirmed cases of esophageal (squamous cell carcinoma and adenocarcinoma), gastro-esophageal junction, colorectal cancers and corresponding normal tissues freshly resected from a cohort of 135 patients, by Western Blotting and Immunofluorescence Staining. The protein is present in its phosphorylated form in cells and tissues. Depending on the extent of phosphorylation it is either present in hyper-phosphorylated (M. Wt. 72 kDa) form or in hypo-phosphorylated form (M. Wt. 68 kDa and 65 kDa). A thorough analysis revealed that expression of E3B1/ABI-1 protein is significantly decreased in esophageal, gastro-esophageal junction and colorectal carcinomas irrespective of age, gender, dietary and smoking habits of the patients. The decrease in expression of E3B1/ABI-1 was consistently observed for all the three isoforms. However, the decrease in the expression of isoforms varied with different forms of cancers. Down-regulation of E3B1/ABI-1 expression in human carcinomas may play a critical role in tumor progression and in determining disease prognosis.