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Disease Markers
Volume 35, Issue 4, Pages 243–247
http://dx.doi.org/10.1155/2013/107470
Research Article

Association of IL-4-590 C>T and IL-13-1112 C>T Gene Polymorphisms with the Susceptibility to Type 2 Diabetes Mellitus

1Genetics Unit, Children Hospital, Mansoura University, Egypt
2Zoology Department, Faculty of Science, Mansoura University, Egypt
3Diabetes and Endocrinology Unit, Specialized Medicine Hospital, Mansoura University, Egypt
4College of Medicine, Qassim University, P.O. Box 5566, Buraydah 51432, Saudi Arabia

Received 18 June 2013; Revised 5 August 2013; Accepted 17 August 2013

Academic Editor: Xiaohong Li

Copyright © 2013 Afaf Alsaid et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. The goal of the study is to investigate the association of IL-4-590 and IL-13-1112 genetic polymorphisms with type 2 diabetes mellitus (T2DM) in Egyptian patients. Subjects and Methods. The study included 135 cases with T2DM and 75 healthy unrelated age-matched controls from the same locality of Egypt. DNA was extracted and processed by the ARMS-PCR technique for characterization of genetic variants of IL-4-590 C>T and IL-13-1112 C>T polymorphisms. Results. Egyptian cases with T2DM showed a lower frequency of the IL-4-590 CC homozygous genotype compared to controls (10.4% versus 43.48%) with a higher CT heterozygous genotype (85.2% versus 47.8%). Similarly, cases showed a lower frequency of the IL-13-1112 CC genotype (20.7% versus 56.8%) with a higher frequency of the heterozygous IL-13-1112 CT genotype (76.3% versus 41.3%). Both polymorphisms showed significantly positive associations with T2DM in the dominant, codominant, and overdominant models of inheritance. On the other hand, comparing genotypes of subgroups related to gender, positive family history, and positive consanguinity showed a nonsignificant difference ( ). Conclusion. Heterozygous genotypes (IL-4-590 CT and IL-13-1112 CT) could be considered as risk factors, while the homozygous wild types (-590 CC and -1112 CC) might be considered protective to T2DM.