Development and Validation of an ELISA at Acidic pH for the Quantitative Determination of IL-13 in Human Plasma and Serum

<table>NSB obtained in plasma samples at pH 7.4, pH 5, and pH 3.5. NSB is the signal obtained by the detection (biotinylated anti-IL13 antibody and enzyme linked streptavidin) of plasma components nonspecifically bound to the plate surface in the absence of capture antibody.</table>

Disease Markers

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Figure 2

Figure 2: Development and Validation of an ELISA at Acidic pH for the Quantitative Determination of IL-13 in Human Plasma and Serum 