Development and Validation of an ELISA at Acidic pH for the Quantitative Determination of IL-13 in Human Plasma and Serum

<table>Recovery of rhIL-13 concentration in the presence of its receptor rhIL-13R<i>α</i>2 as a function of incubation pH. rhIL-13 (10 pg/mL) and its receptor were spiked in a human serum. IL-13 concentration was then measured by ELISA at pH 5 and pH 3.5.</table>

Disease Markers

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Figure 4

Figure 4: Development and Validation of an ELISA at Acidic pH for the Quantitative Determination of IL-13 in Human Plasma and Serum 