Development and Validation of an ELISA at Acidic pH for the Quantitative Determination of IL-13 in Human Plasma and Serum

<table>Recovery of endogenous IL-13 concentration in the presence of rhIL-13R<i>α</i>2 as a function of incubation pH. IL-13 was measured in two human serums spiked with rhIL-13R<i>α</i>2. The IL-13 concentration measured in the absence of spiked rhIL-13R<i>α</i>2 was taken as reference for the calculation of IL-13 recovery.</table>

Disease Markers

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Figure 5

Figure 5: Development and Validation of an ELISA at Acidic pH for the Quantitative Determination of IL-13 in Human Plasma and Serum 