Development and Validation of an ELISA at Acidic pH for the Quantitative Determination of IL-13 in Human Plasma and Serum

Table 1

Background signal and detection limit of rhIL-13 obtained at different incubation pH values.

	pH 7.4	pH 5	pH 3.5	pH 3.25
Background signal (OD495)	0.260	0.115	0.108	0.105
Detection limit (pg/mL)	0.31	0.16	0.31	5.00