Research Article
Development and Validation of an ELISA at Acidic pH for the Quantitative Determination of IL-13 in Human Plasma and Serum
Table 2
Typical IL-13 calibration curve data obtained during assay validation.
| IL-13 standard concentration (pg/mL) | Wells | OD | OD mean | CV (%) | BackCalc mean | BackCalc Cv in % | Accuracy (%) | Accuracy mean (%) |
| 40 | A11 | 3.038 |
3.073 |
1.6 |
39.6 |
2.86 | 97.05 |
99.05 | A12 | 3.108 | 101.06 | 30 | B11 | 2.543 |
2.592 |
2.6 |
30.2 |
3.9 | 97.78 |
100.56 | B12 | 2.64 | 103.33 | 20 | C11 | 1.897 |
1.926 |
2.1 |
20.2 |
2.65 | 99.27 |
101.16 | C12 | 1.955 | 103.06 | 10 | D11 | 1.024 |
1.058 |
4.5 |
10.1 |
5.04 | 97.07 |
100.66 | D12 | 1.091 | 104.25 | 5 | E11 | 0.556 |
0.559 |
0.5 |
4.9 |
0.62 | 97.48 |
97.91 | E12 | 0.561 | 98.34 | 2.5 | F11 | 0.333 |
0.328 |
1.8 |
2.5 |
2.46 | 102.45 |
100.7 | F12 | 0.324 | 98.94 | 1.25 | G11 | 0.21 |
0.213 |
1.5 |
1.3 |
2.93 | 98.51 |
100.6 | G12 | 0.215 | 102.68 | 0.63 | H11 | 0.161 |
0.159 |
1.6 |
0.6 |
4.81 | 104.68 |
101.23 | H12 | 0.158 | 97.79 | 0.31 | D9 | 0.135 |
0.137 |
2.2 |
0.3 |
11.35 | 102.43 |
111.37 | D10 | 0.139 | 120.31 | 0.21 | E9 | 0.124 |
0.125 |
0.7 |
0.2 |
6.75 | 83.33 |
87.51 | E10 | 0.126 | 91.69 | 0.16 | F9 | 0.123 |
0.123 |
0.5 |
0.1 |
5.82 | 96.95 |
93.12 | F10 | 0.122 | 89.29 |
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