Development and Validation of an ELISA at Acidic pH for the Quantitative Determination of IL-13 in Human Plasma and Serum

Table 2

Typical IL-13 calibration curve data obtained during assay validation.

IL-13 standard concentration (pg/mL)	Wells	OD	OD mean	CV (%)	BackCalc mean	BackCalc   Cv in %	Accuracy (%)	Accuracy mean (%)
40	A11	3.038	3.073	1.6	39.6	2.86	97.05	99.05
	A12	3.108					101.06
30	B11	2.543	2.592	2.6	30.2	3.9	97.78	100.56
	B12	2.64					103.33
20	C11	1.897	1.926	2.1	20.2	2.65	99.27	101.16
	C12	1.955					103.06
10	D11	1.024	1.058	4.5	10.1	5.04	97.07	100.66
	D12	1.091					104.25
5	E11	0.556	0.559	0.5	4.9	0.62	97.48	97.91
	E12	0.561					98.34
2.5	F11	0.333	0.328	1.8	2.5	2.46	102.45	100.7
	F12	0.324					98.94
1.25	G11	0.21	0.213	1.5	1.3	2.93	98.51	100.6
	G12	0.215					102.68
0.63	H11	0.161	0.159	1.6	0.6	4.81	104.68	101.23
	H12	0.158					97.79
0.31	D9	0.135	0.137	2.2	0.3	11.35	102.43	111.37
	D10	0.139					120.31
0.21	E9	0.124	0.125	0.7	0.2	6.75	83.33	87.51
	E10	0.126					91.69
0.16	F9	0.123	0.123	0.5	0.1	5.82	96.95	93.12
	F10	0.122					89.29