Regulation of Breast Cancer and Bone Metastasis by MicroRNAs

Table 3

Targets of oncogenic/tumor suppressor miRNAs and their role in cancer cells.

miRNA	Target gene	Techniques	Short description	References
miR-205	ERBB3	WB, LA	Over expression of miR-205 down regulates breast tumors by directly targeting HER3 receptor and inhibits the activation of the downstream mediator Akt.	[125]
	ZEB1	LA	miR-205 is down regulated in cells that had undergone epithelial mesenchymal transition in response to TGF-β. It down regulates E-cadherin transcriptional repressors ZEB1 and enhances tumor metastasis.	[144]
	ZEB2	RT-PCR	miR-205 down regulates E-cadherin and reduces cell locomotion and invasion and exerts a tumor suppressive effect.	[145]
	ZEB2	LA	miR-205 down regulates cells that had undergone epithelial mesenchymal transition in response to transforming growth factor (TGF-β). They down regulate E-cadherin transcriptional repressors ZEB2 and enhance tumor metastasis.	[144]
	VEGFA	LA	miR-205 inhibits cell proliferation and anchorage-independent growth, as well as cell invasion by down regulating transcription of VEGFA.	[72]
	PRKCE	Immunostaining, M	miR-205 down regulates protein kinase C, epsilon and reduces cell locomotion and invasion and exerts a tumor suppressive effect.	[145]
	ZEB1, ZEB2	RT-PCR, WB	Mel-8 suppresses expression of miR-205 which down regulates ZEB1 and ZEB2 resulting in reduced migration and invasion.	[128]
miR-27a	SP1,3,4	WB	Expression of miR-27a up regulates SP1 by down regulating zinc finger ZBTB10 gene, a putative Sp repressor transcriptionally.	[132]
	FOXO1	WB	Several miRNAs are overexpressed in endometrial cancer and they repress FOXO1, a tumor suppressor expression, resulting in G1 cell cycle arrest and cell death.	[146]
	FBXW7	WB, LA	miR-27a overexpression promotes cell growth by suppressing Fbxw7 for viral oncoprotein ST-induced malignant transformation.	[147]
miR-27b	CYP1B1	WB, LA	miR-27b suppresses expression of CYP1B1 in breast cancer and inhibits tumor.	[135]
	ST14	WB, RT-PCR, LA	ST14 reduces cell proliferation, migration, and invasion. miR-27b expression increases during cancer progression, paralleling a decrease in ST14 expression. ST14 reduces cell growth through its effects on cell cycle-related proteins.	[148]
	MMP13	ELISA, LA	IL-1β -induces activation of signal transduction pathways associated with the expression of MMP-13 and down regulated expression of miR-27b in chondrocytes.	[149]
	ZBTB10	WB, RT-PCR	miR-27 down regulates expression of ZBTB10 and increases tumor size, lymph node metastasis, and distant metastasis.	[150]

WB: Western Blot, RT-PCR: Real Time-PCR, LA: Luciferase Assay, M: Microarray.