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Disease Markers
Volume 35 (2013), Issue 5, Pages 581–588
Research Article

Mutation of NPM1 and FLT3 Genes in Acute Myeloid Leukemia and Their Association with Clinical and Immunophenotypic Features

1National Institute of Pathology, Indian Council of Medical Research, Safdarjung Hospital Campus, New Delhi 110029, India
2Department of Hematology, Safdarjung Hospital, New Delhi 110029, India

Received 10 April 2013; Revised 1 October 2013; Accepted 2 October 2013

Academic Editor: Benoit Dugue

Copyright © 2013 Pradeep Singh Chauhan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary figure 1(a) shows the capillary electrophoresis of NPM1 mutation in AML samples run on the ABI 3130xl Genetic Analyzer (16-cm capillaries). Fluorescent PCR product analysis of exon 12 showed one wild type NPM1 peak at 198 bp and additional peak at 202 bp in patients with NPM1 mutation. Figure 1(b) shows the sequence electropherogram of NPM1 mutation generated by direct sequencing of PCR product from normal and NPM1 mutant samples. Reverse PCR primer was used as a sequencing primer. The boxed tetranucleotides shown in the mutant sequence are the 4 bp inserted between nucleotide 960 and 961 from the transcription start site

  1. Supplementary Figure