Research Article

Is Human Papillomavirus Associated with Prostate Cancer Survival?

Figure 1

Tests for specificity of the analysis. Preabsorption test to verify the specificity of the E7 signal on prostate cancer samples. Immunostaining with the Cervimax IHC kit in prostate cancer tissue (a) and after preabsorption with the specific oncoviral peptide (b). Original magnification: 20x; conventional immunohistochemistry performed with diaminobenzidine as a chromogen and hematoxylin for counterstaining. PCR amplification of HPV DNA is reported in (c): MW molecular weight marker, 1–22 amplification carried out in prostate cancer specimens. In 19 cases positive for HPV E7 IHC, 18 were PCR positive and 1 was PCR negative (sample 10). Of those successfully sequenced samples were 4, 7, 8, 9, 13, 14, 15, 17, and 21. Sequencing failed in samples 1, 2, 3, 5, and 6. Samples 12, 16, and 20 refer to amplification carried out in prostate cancer cases negative for HPV E7 immunodetection.