Table of Contents Author Guidelines Submit a Manuscript
Disease Markers
Volume 35 (2013), Issue 6, Pages 701–710
http://dx.doi.org/10.1155/2013/762685
Research Article

Investigation of Genetic Polymorphisms Related to the Outcome of Radiotherapy for Prostate Cancer Patients

1Programa de Mestrado em Genética, Pontifícia Universidade Católica de Goiás, Avenida Universitária 1440 Setor Universitário, 74605-010 Goiânia, GO, Brazil
2Laboratório de Oncogenética e Radiobiologia, Associação de Combate ao Câncer em Goiás, Rua 239 N.52 Lt.29, Setor Universitário, 74605-070 Goiânia, GO, Brazil
3Departamento de Medicina, Pontifícia Universidade Católica de Goiás, Avenida Universitária 1440 Setor Universitário, 74605-010 Goiânia, GO, Brazil
4Serviço de Radioterapia, Hospital Araújo Jorge, Associação de Combate ao Câncer em Goiás, Rua 239 N.52 Lt.181, Setor Universitário, 74605-070 Goiânia GO, Brazil

Received 30 June 2013; Accepted 8 October 2013

Academic Editor: Esperanza Ortega

Copyright © 2013 Hellen Silva Cintra et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The purpose of this study was to evaluate the association between ATM, TP53 and MDM2 polymorphisms in prostate cancer patients and morbidity after radiotherapy. The presence of ATM (rs1801516), TP53 (rs1042522, rs1800371, rs17878362, rs17883323, and rs35117667), and MDM2 (rs2279744) polymorphisms was assessed by direct sequencing of PCR fragments from 48 patients with histologically proven prostate adenocarcinoma and treated with external beam radiation. The side effects were classified according to the Radiation Therapy Oncology Group (RTOG) score. The results showed no association between clinical characteristics and the development of radiation toxicities (P > 0.05). The C>T transition in the position 16273 (intron 3) of TP53 (rs35117667) was significantly associated with the risk of acute skin toxicity (OR: 0.0072, 95% CI 0.0002–0.227, P = 0.003). The intronic TP53 polymorphism at position 16250 (rs17883323) was associated with chronic urinary toxicity (OR: 0.071, 95%CI 0.006–0.784, P = 0.032). No significant associations were found for the remaining polymorphisms (P > 0.05). The results show that clinical characteristics were not determinant on the developing of radiation sensitivity in prostate cancer patients, and intronic TP53 polymorphisms would be associated with increased acute and chronic radiation toxicities. These observations corroborate the importance of investigating the genetic profile to predict adverse side effects in patients undergoing radiotherapy.