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Disease Markers
Volume 34 (2013), Issue 6, Pages 419-424
http://dx.doi.org/10.3233/DMA-130972

Utility of OCT3/4, TSPY and β-Catenin as Biological Markers for Gonadoblastoma Formation and Malignant Germ Cell Tumor Development in Dysgenetic Gonads

Icela Palma,1,2 Nayely Garibay,3 Rocio Pena-Yolanda,4 Alejandra Contreras,5 Atlantida Raya,6 Carolina Dominguez,7 Mirna Romero,1 Gerardo Aristi,8,9 and Gloria Queipo3,8

1Molecular and Cellular Morphology Laboratory, Escuela Superior de Medicina, Instituto Politécnico Nacional, Mexico City, Mexico
2Morphology Department, Facultad de Medicina Veterinaria y Zootecnia, UNAM, Mexico City, Mexico
3Human Genetics Department, Hospital General de México, Mexico City, Mexico
4Pathology Department, Hospital Infantil de México-Federico Gómez, Mexico City, Mexico
5Biology Development Department, Hospital Infantil de México-Federico Gómez, Mexico City, Mexico
6Urology Department, Hospital Infantil de México- Federico Gómez, Mexico City, Mexico
7Endocrinology Department, Hospital Infantil de México-Federico Gómez, Mexico City, Mexico
8Facultad de Medicina Universidad Nacional Autónoma de México, Mexico City, Mexico
9Pathology Department, Hospital General de México, Mexico City, Mexico

Received 5 February 2013; Accepted 5 February 2013

Copyright © 2013 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

BACKGROUND: Gonadoblastoma (GB) is regarded as an in situ form of germ cell tumor in dysgenetic gonads, and 30% of patients with GB develop a dysgerminoma/seminoma tumor.

OBJECTIVE: Determine whether OCT3/4 and β-catenin are expressed in dysgenetic gonads before GB development and whether TSPY participates in the OCT3/4-β-catenin pathways in the malignant invasive behavior.

METHODS: dysgenetic gonads of Disorders of sex differentiation (DSD) patients with mixed gonadal dysgenesis were analyzed by immunohistochemistry and immunofluorescence for comparison with GB and dysgerminoma/seminoma.

RESULTS: Our results suggest that the development of GB is secondary to the interaction of OCT3/4 and TSPY, that β-catenin does not participate in this process.

CONCLUSIONS: The use of this biological markers detects the potential high risk gonads.