Research Article
Altered Peptidase Activities in Thyroid Neoplasia and Hyperplasia
Table 1
Substrates and the reaction conditions for each enzymatic assay.
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Reaction buffers: Phosphate buffer (50 mM) was used for PEP, PGI, APB, APN/CD13, and AlaAP. Tris-HCl (50 mM) was used for APA, AspAP, DPPIV/CD26, CAP, and NEP. b40 μM of the AlaAP inhibitor puromycin was added; c4.6 nM of the angiotensin-converting enzyme inhibitor captopril was added. |