Aquaporin-1 Protein Levels Elevated in Fresh Urine of Renal Cell Carcinoma Patients: Potential Use for Screening and Classification of Incidental Renal Lesions
Figure 1
Western blot analysis of urinary AQP1 in RCC patients. (a) AQP1 in fresh urine collected from eleven patients suspected of RCC and undergoing nephrectomy or partial nephrectomy. The (–) control was protein isolated from the fresh urine of a patient with no known renal masses, malignant or benign, and no other known urological carcinomas. All samples were normalized to creatinine prior to electrophoresis. The reported molecular weight of AQP1 is 28 kD; L denotes the loading control ladder with which sample size was determined. Data are representative of 3 individual Western blots. (b) AQP1 in archived urine, prenephrectomy, of known clear cell renal cell carcinoma patients. (+) control sample is protein isolated from the fresh urine of patient SS056. (c) AQP1 in fresh urine collected from eleven volunteers with no evidence of renal disease, renal injury, or any other urological malignancy. Data are representative of 3 individual Western blots.
