MicroRNA-21 Promotes Cell Growth and Migration by Targeting Programmed Cell Death 4 Gene in Kazakh’s Esophageal Squamous Cell Carcinoma

Figure 2

miR-21 promoted tumor cell migration after transfection. (a) Cell migration ability was assessed using wound-healing assay. Eca109 cells were transfected with miR-21 mimics, inhibitor, and scrambled sequence and were assessed for migration by wound-healing assay at 0 h, 24 h, 48 h, and 72 h after transfection. Images of migratory cells from the scratched boundary were observed and acquired with light microscope (10 × 10). Similar results were obtained in three independent experiments, and shown were representative figures. (b) Statistical analysis of wound-healing. There was significant difference between miR-21 mimics and control group ().

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