Research Article
Variability of the Transferrin Receptor 2 Gene in AMD
Table 1
Sequences of primers and restriction enzymes for genotyping of the c.−258+123T>C and c.1892C>T polymorphisms of the TRF2 gene.
| | Polymorphism | Sequences of primers |
|
c.−258+123T>C
(rs4434553) | Flanking |
F: 5′ GATCACATGGGTTTCTACCTCTTT
R: 5′ CTTAGGTCTCAGGGGAGCAG | PCRa 375 bp | | ASO |
R-A spec: 5′ GACCTGAGTAGGAGCTGATGTTC
R-G spec: 5′ GACCTGAGTAGGAGCTGATGTTT | ASOb 224 bp |
| | | Sequences of primers | Enzyme
(target allele) | Target sequence | Genotypesc |
| | c.1892C>T (rs2075674) | F: 5′-ACTCCCTGCCGTCGAGTTCT-3′
R: 5′-ACTCCCAGAGACCAAGAGCG-3′ | MspA1I (T) | 5′…C(A/C)G↓C(G/T)G…3′
3′…G(T/G)C↑G(C/A)C…3′ | CC 381
CT 381/198/193
TT 198/193 |
|
|
F: forward primer. R: reverse primer. aPCR product length using only flanking primers. bPCR product length with ASO reverse primers. cRestriction products lengths (bp) for each genotype.
|
