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Disease Markers
Volume 2014, Article ID 597892, 7 pages
Research Article

Evaluation of Oxidative Stress Biomarkers in Patients with Fixed Orthodontic Appliances

1Faculty of Dentistry, Department of Orthodontics, Atatürk University, 25240 Erzurum, Turkey
2Department of Periodontology, Oral Health Center, Gülhane Military Hospital, 06020 Ankara, Turkey
3Department of Biochemstry, Faculty of Medical, Atatürk University, 25240 Erzurum, Turkey
4Department of Orthodontics, Faculty of Dentistry, Osman Gazi University, 26140 Eskişehir, Turkey
5Department of Periodontology, Faculty of Dentistry, Atatürk University, 25240 Erzurum, Turkey

Received 10 January 2014; Accepted 14 March 2014; Published 10 April 2014

Academic Editor: Fabrizia Bamonti

Copyright © 2014 Sevil Sema Atuğ Özcan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Aim. The aim of this study was to examine the changes in the levels of interleukine-1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), malondialdehyde (MDA), nitric oxide (NO), and 8-hydroxydeoxyguanosine (8-OHdG) in saliva and IL-1β, TNF-α, and NO in gingival crevicular fluid (GCF) samples of patients with fixed orthodontic appliances. Material and Method. The subject population consisted of 50 volunteers who were in need of orthodontic treatment with fixed orthodontic appliances. GCF and saliva samples were obtained from all individuals before treatment, at 1st month of treatment and at 6th month of treatment. Periodontal clinical parameters were measured. Samples were investigated to detect IL-1β, TNF-α, and 8-OHdG levels using ELISA method and NO and MDA levels using spectrophotometric method. Results. Since IL-1β level detected in GCF at the 6th month of orthodontic treatment is statistically significant according to baseline (), all other biochemical parameters detected both in saliva and in GCF did not show any significant change at any measurement periods. Conclusion. Orthodontic tooth movement and orthodontic materials used in orthodontic treatment do not lead to a change above the physiological limits that is suggestive of oxidative damage in both GCF and saliva.