Mass Spectrometric Screening of Ovarian Cancer with Serum Glycans
Table 3
Candidate biomarkers with , detection frequency, mean intensities, values, and AUC in the training set.
Fractiona
Marker type
Theoretical
Measured
Δ (10−3 Da)
Composition
Frequency
Intensity
P value
AUCc
Average
SDb (10−3 Da)
Hex
Hex NAc
Fuc
Neu AC
Normal
Patient
10%
N-glycan
1501.5293
1501.5222
3.5
7.1
4
4
0
0
100%
100%
14.28 ± 4.48
10.86 ± 3.76
0.72
1647.5873
1647.5916
2.2
4
4
1
0
100%
100%
70.34 ± 8.91
63.17 ± 9.82
0.71
1663.5823
1663.5794
2.4
2.9
5
4
0
0
100%
100%
8.93 ± 2.35
6.15 ± 2.13
0.81
1809.6403
1809.6413
1.8
5
4
1
0
100%
100%
9.34 ± 2.25
6.68 ± 3.06
0.77
1866.6613
1866.6568
2.5
4.5
5
5
0
0
100%
100%
2.03 ± 0.47
1.65 ± 0.42
0.73
2012.7193
2012.7207
1.6
5
5
1
0
100%
100%
9.61 ± 1.85
7.86 ± 1.87
0.74
Glycan
1467.4853
1467.5312
3.7
7
0
2
0
100%
100%
1.15 ± 0.31
1.43 ± 0.43
0.69
Nonglycan
1384.4855
2.6
100%
100%
1.85 ± 0.64
2.46 ± 0.87
0.71
20%
N-glycan
1444.5073
1444.5009
1.9
6.4
4
3
1
0
100%
100%
3.57 ± 0.40
3.06 ± 0.50
0.78
1485.5343
1485.5305
1.3
3.8
3
4
1
0
100%
100%
63.17 ± 13.99
78.05 ± 21.98
0.72
1663.5823
1663.5646
3.6
17.8
5
4
0
0
100%
100%
3.94 ± 0.93
3.08 ± 0.62
0.82
1809.6403
1809.6399
0.5
0.4
5
4
1
0
100%
100%
98.55 ± 12.93
84.32 ± 20.20
0.72
Glycan
1483.4803
1483.5146
3.7
8
0
1
0
100%
100%
0.86 ± 0.21
1.17 ± 0.49
0.68
1524.5073
1524.4894
3.8
17.9
7
1
1
0
99%
99%
0.95 ± 0.21
0.72 ± 0.25
0.76
1540.5023
1540.4550
3.7
47.3
8
1
0
0
100%
100%
2.04 ± 0.58
1.40 ± 0.56
0.79
1629.5383
1629.5791
3.3
8
0
2
0
100%
100%
2.70 ± 0.37
2.39 ± 0.42
0.72
1791.5913
1791.6336
3.7
9
0
1
0
100%
100%
1.48 ± 0.24
1.06 ± 0.32
0.85
Nonglycan
1280.4357
2.7
100%
100%
2.34 ± 0.36
2.08 ± 0.38
0.69
1442.4848
3.4
100%
100%
1.21 ± 0.19
0.90 ± 0.23
0.84
1502.5077
4.8
100%
100%
3.66 ± 0.53
3.15 ± 0.50
0.75
1518.4722
5.6
100%
98%
0.87 ± 0.37
0.59 ± 0.26
0.75
1562.4391
7.2
100%
98%
0.67 ± 0.15
0.52 ± 0.14
0.77
1708.5716
5.5
100%
100%
2.67 ± 0.51
1.93 ± 0.62
0.82
1945.6596
5.1
100%
100%
1.92 ± 0.30
1.36 ± 0.46
0.84
Acetonitrile/H2O fraction in the elution of glycans from graphitized carbon cartridges. The standard deviation of each in the training set was calculated and expressed in 10−3 Da. AUC of the biomarkers selected for the use in the later blind test set is in bold.
