Inhibition of HIF-1α Affects Autophagy Mediated Glycosylation in Oral Squamous Cell Carcinoma Cells

Figure 2

Protein expression of O-GlcNAc, OGT, and OGA changes in HIF-1α inhibition. (a) Lysates from Tca8113 cells treated with HIF-1α inhibitors were analyzed by western blotting using O-GlcNAc, OGA, OGT, and GAPDH antibodies. (b) Quantification of O-GlcNAc, OGT, and OGA (, Student’s -test). The data are presented as mean ± SD for three independent experiments. (c) Western blots showing Tca8113 cells were treated with 25 μM PX-478 for 0, 4, 6, and 8 h. (d) Quantification of the amount of OGT bands in (c) (, Student’s -test).

(a)

(b)

(c)

(d)