Inhibition of HIF-1α Affects Autophagy Mediated Glycosylation in Oral Squamous Cell Carcinoma Cells

Figure 3

Atg12 siRNA and Atg1 siRNA transfection increase O-GlcNAc and OGT in Tca8113 cells. (a) After transfection with ATG12 and ATG1 siRNA for 48 h, the protein levels of ATG12, ATG1, LC3-II, and LC3-I were analyzed by western blotting. (b) Quantifications of protein amount in (a) separately (, Student’s -test). (c) Lysates from Tca8113 cells transfected with ATG12 and Atg1 siRNA were analyzed by western blotting using O-GlcNAc, OGT, and GAPDH antibodies. (d) Quantification of protein amount in (c) (, Student’s -test).

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