Inhibition of HIF-1α Affects Autophagy Mediated Glycosylation in Oral Squamous Cell Carcinoma Cells

Figure 4

Atg12 and Atg1 siRNA restore protein level of O-GlcNAc and OGT under PX-478 treatment. (a) The expression of OGT in 0, 8, 16, 24, 36, and 48 h after treatment of Atg12 and Atg1 siRNA and PX-478. (b) Quantifications of protein amount in (a) (, Student’s -test). (c) LC3-I and LC3-II expression in the 48 hours in Atg12 and Atg1 siRNA and PX-478 treated Tca8113 cells in immunofluorescence assay (400x).

(a)

(b)

(c)