Polymorphisms rs12998 and rs5780218 in KiSS1 Suppressor Metastasis Gene in Mexican Patients with Breast Cancer

Table 2

Primer sequences, PCR conditions, and restriction enzymes (RE) used for genotyping rs12998 and rs5780218 polymorphisms in KiSS1 gene. AT: annealing temperature.

Polymorphism	Primers sequence	AT; (°C)	Amplicon; (bp)	RE
rs 12998 A/G	F: 5′-ACT TgC TCA CAT TCC ACA gg-3′; R: 5′-gCA TCT CTC TgC TCT TgC AC-3′	65	238	Nla IV
rs 5780218 —/A	F: 5′-CCT TTg CCT gCC Tgg ATg CA-3′; R: 5′-Tgg gCC TgT gCT Tgg AgA Cg-3′	65	294	Sml I