Krüppel-Like Factor 4 Inhibits the Transforming Growth Factor-β1-Promoted Epithelial-to-Mesenchymal Transition via Downregulating Plasminogen Activator Inhibitor-1 in Lung Epithelial Cells

Figure 1

TGF-β downregulates KLF-4 in both mRNA and protein levels in mouse lung epithelial LA-4 cells. (a) KLF-4 mRNA level in LA-4 cells, which were treated with 0, 1, 2, 5, or 10 ng/mL TGF-β for 12 hours. (b) KLF-4 mRNA level in LA-4 cells after the treatment with 5 ng/mL TGF-β for 0, 1, 3, 6, or 12 hours. (c) Western blot analysis of KLF-4 in LA-4 cells after the treatment with 0, 1, 2, 5, or 10 ng/mL TGF-β for 24 hours; each KLF-4 value was expressed as percentage to β-actin. (d) KLF-4 in protein level in LA-4 cells after treatment with 5 ng/mL TGF-β for 0, 6, 12, 24, or 48 hours. Each piece of data was averaged for triple independent experiments. HPT: hours posttreatment, , , , or ns: no significance.

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