Krüppel-Like Factor 4 Inhibits the Transforming Growth Factor-β1-Promoted Epithelial-to-Mesenchymal Transition via Downregulating Plasminogen Activator Inhibitor-1 in Lung Epithelial Cells

Figure 3

PAI-1 knockdown inhibits the TGF-β-promoted EMT in LA-4 cells. (a) PAI-1 mRNA level in LA-4 cells which were transfected with 20 or 40 nM PAI-1-specific siRNA (siRNA-PAI-1) or with control siRNA (siRNA-Con) for 12 hours. (b) Western blot analysis of PAI-1 in LA-4 cells which were transfected with 20 or 40 nM siRNA-PAI-1 or with siRNA-Con for 24 hours. (c) Western blot analysis of PAI-1, E-cadherin, and Collagen I in LA-4 cells which were transfected with 20 or 40 nM siRNA-PAI-1 or with siRNA-Con and were treated with 5 ng/mL TGF-β for 24 hours. (d) and (e) Relative levels of PAI-1 (d) or E-cadherin and Collagen I (e) in the siRNA-PAI-transfected and the TGF-β-treated LA-4 cells. All results were shown as values ± SE for three independent experiments. , , , or ns: no significance.

(a)

(b)

(c)

(d)

(e)