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Disease Markers
Volume 2015, Article ID 479251, 10 pages
http://dx.doi.org/10.1155/2015/479251
Research Article

Blood Contamination in Saliva: Impact on the Measurement of Salivary Oxidative Stress Markers

1Institute of Molecular Biomedicine, Faculty of Medicine, Comenius University, Sasinkova 4, 811 08 Bratislava, Slovakia
2Department of Stomatology and Maxillofacial Surgery, Comenius University, Heydukova 10, 812 50 Bratislava, Slovakia
3Center for Molecular Medicine, Slovak Academy of Sciences, Vlárska 7, 831 01 Bratislava, Slovakia
4Institute of Pathophysiology, Faculty of Medicine, Comenius University, Sasinkova 4, 811 08 Bratislava, Slovakia
5Department of Molecular Biology, Faculty of Natural Sciences, Comenius University, Mlynská Dolina, 842 15 Bratislava, Slovakia

Received 5 March 2015; Accepted 8 July 2015

Academic Editor: Kishore Chaudhry

Copyright © 2015 Natália Kamodyová et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Salivary oxidative stress markers represent a promising tool for monitoring of oral diseases. Saliva can often be contaminated by blood, especially in patients with periodontitis. The aim of our study was to examine the impact of blood contamination on the measurement of salivary oxidative stress markers. Saliva samples were collected from 10 healthy volunteers and were artificially contaminated with blood (final concentration 0.001–10%). Next, saliva was collected from 12 gingivitis and 10 control patients before and after dental hygiene treatment. Markers of oxidative stress were measured in all collected saliva samples. Advanced oxidation protein products (AOPP), advanced glycation end products (AGEs), and antioxidant status were changed in 1% blood-contaminated saliva. Salivary AOPP were increased in control and patients after dental treatment (by 45.7% and 34.1%, ). Salivary AGEs were decreased in patients after microinjury (by 69.3%, ). Salivary antioxidant status markers were decreased in both control and patients after dental treatment ( and ). One % blood contamination biased concentrations of salivary oxidative stress markers. Saliva samples with 1% blood contamination are visibly discolored and can be excluded from analyses without any specific biochemic detection of blood constituents. Salivary markers of oxidative stress were significantly altered in blood-contaminated saliva in control and patients with gingivitis after dental hygiene treatment.