Allelic Imbalance of mRNA Associated with α2-HS Glycoprotein (Fetuin-A) Polymorphism

Figure 2

Allele-specific PCR amplification. (a) Nondenaturing polyacrylamide gel electrophoresis of allele-specific RT-PCR products; left: primer set for 1 and right: that for 2. Lane 1: recombinant 1 fragment for template; lane 2: recombinant 2 one for template; lane 3: no template for the negative control. (b) Standard plot for allele-specific real-time PCR amplification. The least-squares regression model was calculated from values of cycle threshold (Ct) versus addition of the recombinant DNA fragment in triplicate.

(a)

(b)