Allelic Imbalance of mRNA Associated with α2-HS Glycoprotein (Fetuin-A) Polymorphism

Figure 3

Two intra-assays of intraindividual assay in the human liver tissues (a) and intracellular assay in three culture cell lines (b and c). (a) Relative amount of 1 mRNA to that of 2 was obtained in genomic DNA (left) and mRNA from the liver tissue (right). Bars represent the mean ± SD of four specimens. (b) Nondenaturing polyacrylamide gel electrophoresis of allele-specific RT-PCR for 1, 2, and β-actin products. Lane 1: HeLa; lane 2: HepG2; and lane 3: PLC/PRF/5. (c) Comparison of allelic mRNA amount of 1 and 2 to β-actin. N.D. denotes not detected. Bars represent the mean ± SD in triplicate; significant versus the amount of 1.

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