Disease Markers / 2016 / Article / Tab 7 / Review Article
Identification of Gingival Crevicular Fluid Sampling, Analytical Methods, and Oral Biomarkers for the Diagnosis and Monitoring of Periodontal Diseases: A Systematic Review Table 7 Comparison of the mean of IL-1β concentration between different studies using the same analytical techniques.
Study [41 ] [12 ] [51 ] [67 ] [103 ] ELISA kit Standard ELISA kit (Bender Med Systems, Vienna, Austria) Standard ELISA kit (Immunotech, France) Standard ELISA kit (RayBiotech) Standard ELISA kit (Quantikine R & D System) Standard ELISA kit (Biosource, Ontario, CA) GCF collection method Paper strips Microcapillary tubes Paper strips Paper strips Paper strips Number of GCF samples 2 20 µ l 4 1 4 Number of subjects 20H, 20G, 20CP, 20GAgP 30H, 30CP 21H, 21G, 21CP, 21GAgP 21H, 30CP 10H, 25G Mean and SD for IL-1β concentration (pg/ml) and clinical parameters for CP patients 128.99 409.2733 (98.0503) 423.65 (35.24) 72.5 (37.0) PD = 5.24 (0.66) OHI-S = 5.1567 (1.4343) PD = 3.45 (0.46) PD = 6.4 (0.6) CAL = 5.44 (0.63) GI = 1.7793 (0.4253) CAL = 3.62 (0.34) CAL = 5.4 (0.9) PI = 3.91 (0.48) PDI = 4.0333 (1.1592) PI = 2.16 (0.28) PI (%) = 64.4 (18.8) PBI = 2.61 (0.32) PD = 4.7667 (1.6333) GI = 2.18 (0.34) GI = 2.3 (0.8) BOP = 2.0333 (0.8899) BOP = 81.24 ± 14.20 Mean and SD for IL-1β concentration (pg/ml) and clinical parameters for G patients 45.68 52.10 (7.15) 17.8732 (10.0523) PD = 2.51 (0.37) PD = 2.36 (0.23) PD = 1.3029 (0.3142) CAL = 0.66 (0.57) CAL = 2.32 (0.18) CAL = 1.3029 (0.3142) PI = 3.71 (0.57) PI = 1.68 (0.24) PI = 0.5563 (0.5410) PBI = 2.16 (0.33) GI = 1.62 ± 0.28 GI = 0.4832 (0.4959) BOP = 71.24 ± 12.40 GBI = 0.1635 (0.1904) Mean and SD for IL-1β concentration (pg/ml) and clinical parameters for H subjects 49.81 195.77 (80.0795) 36.44 (8.86) 15.5 (14.0) 14.0000 (9.9482) PD = 1.5 (0.17) OHI-S = 2.0533 (0.1925) PD = 1.64 (0.42) PD = 1.5 (0.9) PD = 1.0728 (0.0689) CAL = 0.04 (0.06) GI = 0.2333 (0.4302) CAL = 1.74 (0.42) CAL ≤ 1 CAL = 1.0728 (0.0689) PI = 1.41 (0.39) PDI = 0.2333 (0.4302) PI = 1.28 (0.12) PI (%) = 45.8 (12.7) PI = 0.1883 (0.2346) PBI = 0.36 (0.22) PD = 0.0000 (0.0000) GI = 1.25 (0.11) GI = 0.7 (0.3) GI = 0.0000 (0.0000) BOP = 0.0000 (0.0000) BOP = 5.80 (3.50) GBI = 0.0000 (0.0000) Mean and SD for IL-1β concentration (pg/ml) and clinical parameters for GAgP patients 93.78 110.23 (9.20) PD = 5.03 (1.09) PD = 3.83 (0.54) CAL = 5.39 (1.97) CAL = 3.93 (0.27) PI = 3.55 (1.09) PI = 2.42 (0.35) PBI = 2.15 (0.91) GI = 2.31 (0.44) BOP = 86.41 (10.20)
CAL = clinical attachment loss. PBI = papilla bleeding index. OHI-S = simplified oral hygiene index. GI = gingival index. PDI = periodontal disease index. PD = probing depth. BOP = bleeding on probing. GBI = gingival bleeding index. PI = plaque index.