Evaluation of MTBDRplus and MTBDRsl in Detecting Drug-Resistant Tuberculosis in a Chinese Population

Table 1

PCR and DNA sequencing primers.

Locus	Primer	Sequence (5′ to 3′)	Size (bp)	Position	Product (bp)
Rv0577	Rv0577-f	ATGCCCAAGAGAAGCGAATACAGGCAA	27	671166⋯671192	786
	Rv0577-r	CTATTGCTGCGGTGCGGGCTTCAA	24	671951⋯671928	786
16s rRNA	16SrRNA-f	ACGGTGGGTACTAGGTGTGGGTTTC	25	1472650–674	543
	16SrRNA-r	TCTGCGATTACTAGCGACTCCGACTTCA	28	1473192–165	543
rpoB	rpoB-f	CTTGCACGAGGGTCAGACCA	20	760829⋯760848	543
	rpoB-r	ATCTCGTCGCTAACCACGCC	20	761371⋯761352	543
inhA; (promoter)	inhA-f	TGCCCAGAAAGGGATCCGTCATG	23	2154886⋯2154905	455
	inhA-r	ATGAGGAATGCGTCCGCGGA	20	2155340⋯2155321	455
katG	katG-f	AACGACGTCGAAACAGCGGC	20	2154886⋯2154905	455
	katG-r	GCGAACTCGTCGGCCAATTC	20	2155340⋯2155321	455
gyrA	gyrA-f	CCCTGCGTTCGATTGCAAAC	20	7273⋯7292	423
	gyrA-r	CTTCGGTGTACCTCATCGCC	20	7695⋯7676	423
embB	embB-f	CTGACCGACGCCGTGGTGATAT	22	4247345⋯4247366	490
	embB-r	TGAATGCGGCGGTAACGACG	20	4247834⋯4247815	490
rrs	rrs-r	GTCCGAGTGTTGCCTCAGG	19	1473518⋯1473500	516
	rrs-f	GTCAACTCGGAGGAAGGTGG	20	1473003⋯1473022	516
eis	eis-r	GCGTAACGTCACGGCGAAATTC	22	2715477⋯2715456	567
	eis-f	GTCAGCTCATGCAAGGTG	18	2714911⋯2714928	567
gyrB	gyrB-f	AAGACCAAGTTGGGCAACAC	20	6353⋯6372	609
	gyrB-r	CTGCCACTTGAGTTTGTACA	20	6961⋯6942	609