WISP1 Is Increased in Intestinal Mucosa and Contributes to Inflammatory Cascades in Inflammatory Bowel Disease

Figure 1

WISP1 is highly increased in inflamed mucosa and LPMCs of IBD patients. (a) qRT-PCR analysis of WISP1 in colonic samples from healthy controls (HC, ), inflamed mucosa of active Crohn’s disease (CD) patients (A-CD, ), active UC patients (A-UC, ), CD patients in remission (R-CD, ), and UC patients in remission (R-UC, ). (b) and (c) Representative western blots of WISP1 and β-actin in total proteins from colonic samples (b) and LPMCs (c) from HC (), CD patients (), and UC patients (). Right panel in (b) and (c) shows the quantitative analysis of WISP1/β-actin ratio. (d) Relative expression of WISP1 in intestinal mucosa from inflamed and uninflamed intestinal mucosa from the same patients with IBD (). (e) WISP1 expression in different subsets of immune cells. Peripheral blood B cells, CD4⁺ T cells, CD8⁺ T cells, monocytes, and neutrophils (1 × 10⁶ for each subset) were extracted from 10 healthy donors, and WISP1 expression was determined by qRT-PCR. (f) Mucosal biopsies were taken from 13 patients with CD, 12 patients with UC, and 10 healthy controls. The levels of mRNA for WISP1 were detected by qRT-PCR. Gene expression was normalized to GAPDH mRNA levels in each sample. (d) Paired Student’s -test and (a, b, c, and f) one-way ANOVA. , , and ; data are expressed as mean ± s.e.m. for all samples.

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