The Prognostic Role of SIRT1-Autophagy Axis in Gastric Cancer

Figure 2

Representative images of immunohistochemical staining of Beclin-1 and SIRT1 in GC and adjacent NNM tissues. Beclin-1 is expressed in the cytoplasm of epithelial mucosal cells and GC cells. SIRT1 is expressed mainly in the cell nuclei and, to a lesser extent, in the cytoplasm of epithelial mucosal and cancer cells. Scale bar: 20 μm ((a), (d), (e), (h), (i), (l), (m), (p)) and 50 μm ((b), (c), (f), (g), (j), (k), (n), (o)). (a) and (d), (b) and (c): High expression of SIRT1 and Beclin-1 in GC consecutive slides and co-localization. (f) and (g), (e) and (h), (n) and (o), (m) and (p) Low expression of SIRT1 and low expression of Beclin-1 in GC and adjacent NNM consecutive slides and colocalization. (i) and (j) High expression of SIRT1 in adjacent NNM. (k) and (l) High expression of Beclin-1 in adjacent NNM.