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Disease Markers
Volume 2016 (2016), Article ID 9179632, 8 pages
http://dx.doi.org/10.1155/2016/9179632
Review Article

Salivary Markers for Periodontal and General Diseases

School of Dental Medicine, First Faculty of Medicine and General University Hospital, Charles University, Karlovo Namesti 32 and Katerinska 32, 121 11 Prague, Czech Republic

Received 4 December 2015; Revised 7 March 2016; Accepted 15 March 2016

Academic Editor: Shih-Ping Hsu

Copyright © 2016 Stepan Podzimek et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. G. B. Proctor, “The physiology of salivary secretion,” Periodontology 2000, vol. 70, no. 1, pp. 11–25, 2016. View at Publisher · View at Google Scholar
  2. J. A. Loo, W. Yan, P. Ramachandran, and D. T. Wong, “Comparative human salivary and plasma proteomes,” Journal of Dental Research, vol. 89, no. 10, pp. 1016–1023, 2010. View at Publisher · View at Google Scholar · View at Scopus
  3. L. A. S. Nunes, S. Mussavira, and O. S. Bindhu, “Clinical and diagnostic utility of saliva as a non-invasive diagnostic fluid: a systematic review,” Biochemia Medica, vol. 25, no. 2, pp. 177–192, 2015. View at Publisher · View at Google Scholar · View at Scopus
  4. S. P. Barros, R. Williams, S. Offenbacher, and T. Morelli, “Gingival crevicular fluid as a source of biomarkers for periodontitis,” Periodontology 2000, vol. 70, no. 1, pp. 53–64, 2016. View at Publisher · View at Google Scholar
  5. R. R. Wassall and P. M. Preshaw, “Clinical and technical considerations in the analysis of gingival crevicular fluid,” Periodontology 2000, vol. 70, no. 1, pp. 65–79, 2016. View at Publisher · View at Google Scholar
  6. Y. Zhang, J. Sun, C.-C. Lin, E. Abemayor, M. B. Wang, and D. T. W. Wong, “The emerging landscape of salivary diagnostics,” Periodontology 2000, vol. 70, no. 1, pp. 38–52, 2016. View at Publisher · View at Google Scholar
  7. A. Segal and D. T. Wong, “Salivary diagnostics: enhancing disease detection and making medicine better,” European Journal of Dental Education, vol. 12, no. 1, pp. 22–29, 2008. View at Publisher · View at Google Scholar · View at Scopus
  8. J. M. Yoshizawa, C. A. Schafer, J. J. Schafer, J. J. Farrell, B. J. Paster, and D. T. W. Wong, “Salivary biomarkers: toward future clinical and diagnostic utilities,” Clinical Microbiology Reviews, vol. 26, no. 4, pp. 781–791, 2013. View at Publisher · View at Google Scholar · View at Scopus
  9. D. A. Chambers, P. B. Imrey, R. L. Cohen, J. M. Crawford, M. E. Alves, and T. A. McSwiggin, “A longitudinal study of aspartate aminotransferase in human gingival crevicular fluid,” Journal of Periodontal Research, vol. 26, no. 2, pp. 65–74, 1991. View at Publisher · View at Google Scholar · View at Scopus
  10. A. Totan, M. Greabu, C. Totan, and T. Spinu, “Salivary aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase: possible markers in periodontal diseases?” Clinical Chemistry and Laboratory Medicine, vol. 44, no. 5, pp. 612–615, 2006. View at Publisher · View at Google Scholar · View at Scopus
  11. Y. Nomura, Y. Shimada, N. Hanada et al., “Salivary biomarkers for predicting the progression of chronic periodontitis,” Archives of Oral Biology, vol. 57, no. 4, pp. 413–420, 2012. View at Publisher · View at Google Scholar · View at Scopus
  12. I. B. Lamster, E. Kaufman, J. T. Grbic, L. J. Winston, and R. E. Singer, “β-glucuronidase activity in saliva: relationship to clinical periodontal parameters,” Journal of Periodontology, vol. 74, no. 3, pp. 353–359, 2003. View at Publisher · View at Google Scholar · View at Scopus
  13. S. Elgün, N. Özmeriç, and S. Demirtaş, “Alanine aminopeptidase and dipeptidylpeptidase IV in Saliva: the possible role in periodontal disease,” Clinica Chimica Acta, vol. 298, no. 1-2, pp. 187–191, 2000. View at Publisher · View at Google Scholar · View at Scopus
  14. K. Suomalainen, L. Saxén, P. Vilja, and J. Tenovuo, “Peroxidases, lactoferrin and lysozyme in peripheral blood neutrophils, gingival crevicular fluid and whole saliva of patients with localized juvenile periodontitis,” Oral Diseases, vol. 2, no. 2, pp. 129–134, 1996. View at Google Scholar · View at Scopus
  15. B. E. Britigan and B. L. Edeker, “Pseudomonas and neutrophil products modify transferrin and lactoferrin to create conditions that favor hydroxyl radical formation,” The Journal of Clinical Investigation, vol. 88, no. 4, pp. 1092–1102, 1991. View at Publisher · View at Google Scholar · View at Scopus
  16. T. Ingman, T. Tervahartiala, Y. Ding et al., “Matrix metalloproteinases and their inhibitors in gingival crevicular fluid and saliva of periodontitis patients,” Journal of Clinical Periodontology, vol. 23, no. 12, pp. 1127–1132, 1996. View at Publisher · View at Google Scholar
  17. T. Sorsa, L. Tjäderhane, Y. T. Konttinen et al., “Matrix metalloproteinases: contribution to pathogenesis, diagnosis and treatment of periodontal inflammation,” Annals of Medicine, vol. 38, no. 5, pp. 306–321, 2006. View at Publisher · View at Google Scholar · View at Scopus
  18. J. L. Ebersole, J. L. Schuster, J. Stevens et al., “Patterns of salivary analytes provide diagnostic capacity for distinguishing chronic adult periodontitis from health,” Journal of Clinical Immunology, vol. 33, no. 1, pp. 271–279, 2013. View at Publisher · View at Google Scholar · View at Scopus
  19. N. Rathnayake, S. Åkerman, B. Klinge et al., “Salivary biomarkers of oral health—a cross-sectional study,” Journal of Clinical Periodontology, vol. 40, no. 2, pp. 140–147, 2013. View at Publisher · View at Google Scholar · View at Scopus
  20. W. M. Sexton, Y. Lin, R. J. Kryscio, D. R. Dawson III, J. L. Ebersole, and C. S. Miller, “Salivary biomarkers of periodontal disease in response to treatment,” Journal of Clinical Periodontology, vol. 38, no. 5, pp. 434–441, 2011. View at Publisher · View at Google Scholar · View at Scopus
  21. J. S. Kinney, T. Morelli, T. Braun et al., “Saliva/pathogen biomarker signatures and periodontal disease progression,” Journal of Dental Research, vol. 90, no. 6, pp. 752–758, 2011. View at Publisher · View at Google Scholar · View at Scopus
  22. U. K. Gursoy, E. Könönen, S. Huumonen et al., “Salivary type I collagen degradation end-products and related matrix metalloproteinases in periodontitis,” Journal of Clinical Periodontology, vol. 40, no. 1, pp. 18–25, 2013. View at Publisher · View at Google Scholar · View at Scopus
  23. C. A. Meschiari, A. M. Marcaccini, B. C. Santos Moura, L. R. Zuardi, J. E. Tanus-Santos, and R. F. Gerlach, “Salivary MMPs, TIMPs, and MPO levels in periodontal disease patients and controls,” Clinica Chimica Acta, vol. 421, pp. 140–146, 2013. View at Publisher · View at Google Scholar · View at Scopus
  24. T. Sorsa, U. K. Gursoy, S. Nwhator et al., “Analysis of matrix metalloproteinases, especially MMP-8, in gingival crevicular fluid, mouthrinse and saliva for monitoring periodontal diseases,” Periodontology 2000, vol. 70, no. 1, pp. 142–163, 2016. View at Publisher · View at Google Scholar
  25. G. J. Van Steijn, A. V. Nieuw Amerongen, E. C. I. Veerman, S. Kasanmoentalib, and B. Overdijk, “Effect of periodontal treatment on the activity of chitinase in whole saliva of periodontitis patients,” Journal of Periodontal Research, vol. 37, no. 4, pp. 245–249, 2002. View at Publisher · View at Google Scholar · View at Scopus
  26. N. Ozmeric, T. Baydar, A. Bodur et al., “Level of neopterin, a marker of immune cell activation in gingival crevicular fluid, saliva, and urine in patients with aggressive periodontitis,” Journal of Periodontology, vol. 73, no. 7, pp. 720–725, 2002. View at Publisher · View at Google Scholar · View at Scopus
  27. F. S. Lakschevitz, G. M. Aboodi, and M. Glogauer, “Oral neutrophil transcriptome changes result in a pro-survival phenotype in periodontal diseases,” PLoS ONE, vol. 8, no. 7, Article ID e68983, 2013. View at Publisher · View at Google Scholar · View at Scopus
  28. D. L. Korte and J. Kinney, “Personalized medicine: an update of salivary biomarkers for periodontal diseases,” Periodontology 2000, vol. 70, no. 1, pp. 26–37, 2016. View at Publisher · View at Google Scholar
  29. M. Desvarieux, R. T. Demmer, T. Rundek et al., “Relationship between periodontal disease, tooth loss and carotid artery plaque: the oral infections and vascular disease epidemiology study (INVEST),” Stroke, vol. 34, no. 9, pp. 2120–2125, 2003. View at Publisher · View at Google Scholar · View at Scopus
  30. N. J. Park, Y. Li, T. Yu, B. M. N. Brinkman, and D. T. Wong, “Characterization of RNA in saliva,” Clinical Chemistry, vol. 52, no. 6, pp. 988–994, 2006. View at Publisher · View at Google Scholar · View at Scopus
  31. Z. Chen, M. G. Mauk, J. Wang et al., “A microfluidic system for saliva-based detection of infectious diseases,” Annals of the New York Academy of Sciences, vol. 1098, pp. 429–436, 2007. View at Publisher · View at Google Scholar · View at Scopus
  32. W. R. Abrams, C. A. Barber, K. McCann et al., “Development of a microfluidic device for detection of pathogens in oral samples using upconverting phosphor technology (UPT),” Annals of the New York Academy of Sciences, vol. 1098, pp. 375–388, 2007. View at Publisher · View at Google Scholar · View at Scopus
  33. P. L. A. M. Corstjens, Z. Chen, M. Zuiderwijk et al., “Rapid assay format for multiplex detection of humoral immune responses to infectious disease pathogens (HIV, HCV, and TB),” Annals of the New York Academy of Sciences, vol. 1098, pp. 437–445, 2007. View at Publisher · View at Google Scholar · View at Scopus
  34. F. D'Aiuto, M. Parkar, G. Andreou et al., “Periodontitis and systemic inflammation: control of the local infection is associated with a reduction in serum inflammatory markers,” Journal of Dental Research, vol. 83, no. 2, pp. 156–160, 2004. View at Publisher · View at Google Scholar · View at Scopus
  35. I. Ouellet-Morin, A. Danese, B. Williams, and L. Arseneault, “Validation of a high-sensitivity assay for C-reactive protein in human saliva,” Brain, Behavior, and Immunity, vol. 25, no. 4, pp. 640–646, 2011. View at Publisher · View at Google Scholar · View at Scopus
  36. E. Megson, T. Fitzsimmons, K. Dharmapatni, and P. Mark Bartold, “C-reactive protein in gingival crevicular fluid may be indicative of systemic inflammation,” Journal of Clinical Periodontology, vol. 37, no. 9, pp. 797–804, 2010. View at Publisher · View at Google Scholar · View at Scopus
  37. S. Podzimek, J. Mysak, T. Janatova, and J. Duskova, “C-reactive protein in peripheral blood of patients with chronic and aggressive periodontitis, gingivitis, and gingival recessions,” Mediators of Inflammation, vol. 2015, Article ID 564858, 7 pages, 2015. View at Publisher · View at Google Scholar · View at Scopus
  38. Y. Murakami, S. Hanazawa, S. Tanaka, H. Iwahashi, S. Kitano, and S. Fujisawa, “Fibronectin in saliva inhibits Porphyromonas gingivalis fimbria-induced expression of inflammatory cytokine gene in mouse macrophages,” FEMS Immunology and Medical Microbiology, vol. 22, no. 3, pp. 257–262, 1998. View at Publisher · View at Google Scholar · View at Scopus
  39. M. A. Lie, B. G. Loos, Y. M. C. Henskens et al., “Salivary cystatin activity and cystatin C in natural and experimental gingivitis in smokers and non-smokers,” Journal of Clinical Periodontology, vol. 28, no. 10, pp. 979–984, 2001. View at Publisher · View at Google Scholar · View at Scopus
  40. M. S. Rasch, B. L. Mealey, T. J. Prihoda, D. S. Woodard, and L. M. McManus, “The effect of initial periodontal therapy on salivary platelet-activating factor levels in chronic adult periodontitis,” Journal of Periodontology, vol. 66, no. 7, pp. 613–623, 1995. View at Publisher · View at Google Scholar · View at Scopus
  41. V. Booth, S. Young, A. Cruchley, N. S. Taichman, and E. Paleolog, “Vascular endothelial growth factor in human periodontal disease,” Journal of Periodontal Research, vol. 33, no. 8, pp. 491–499, 1998. View at Google Scholar · View at Scopus
  42. M. Ohshima, K. Fujikawa, H. Akutagawa, T. Kato, K. Ito, and K. Otsuka, “Hepatocyte growth factor in saliva: a possible marker for periodontal disease status,” Journal of oral science, vol. 44, no. 1, pp. 35–39, 2002. View at Publisher · View at Google Scholar · View at Scopus
  43. U. K. Gursoy, E. Könönen, P. J. Pussinen et al., “Use of host- and bacteria-derived salivary markers in detection of periodontitis: a cumulative approach,” Disease Markers, vol. 30, no. 6, pp. 299–305, 2011. View at Publisher · View at Google Scholar · View at Scopus
  44. K. M. Jaedicke, P. M. Preshaw, and J. J. Taylor, “Salivary cytokines as biomarkers of periodontal diseases,” Periodontology 2000, vol. 70, no. 1, pp. 164–183, 2016. View at Publisher · View at Google Scholar
  45. P. P. Costa, G. L. Trevisan, G. O. Macedo et al., “Salivary interleukin-6, matrix metalloproteinase-8, and osteoprotegerin in patients with periodontitis and diabetes,” Journal of Periodontology, vol. 81, no. 3, pp. 384–391, 2010. View at Publisher · View at Google Scholar
  46. S. Liskmann, T. Vihalemm, O. Salum, K. Zilmer, K. Fischer, and M. Zilmer, “Correlations between clinical parameters and interleukin-6 and interleukin-10 levels in saliva from totally edentulous patients with peri-implant disease,” The International Journal of Oral and Maxillofacial Implants, vol. 21, no. 4, pp. 543–550, 2006. View at Google Scholar · View at Scopus
  47. N. Spielmann and D. Wong, “Saliva: diagnostics and therapeutic perspectives,” Oral Diseases, vol. 17, no. 4, pp. 345–354, 2011. View at Publisher · View at Google Scholar · View at Scopus
  48. P. Denny, F. K. Hagen, M. Hardt et al., “The proteomes of human parotid and submandibular/sublingual gland salivas collected as the ductal secretions,” Journal of Proteome Research, vol. 7, no. 5, pp. 1994–2006, 2008. View at Publisher · View at Google Scholar · View at Scopus
  49. D. V. Sculley and S. C. Langley-Evans, “Periodontal disease is associated with lower antioxidant capacity in whole saliva and evidence of increased protein oxidation,” Clinical Science, vol. 105, no. 2, pp. 167–172, 2003. View at Publisher · View at Google Scholar · View at Scopus
  50. M. Takane, N. Sugano, H. Iwasaki, Y. Iwano, N. Shimizu, and K. Ito, “New biomarker evidence of oxidative DNA damage in whole saliva from clinically healthy and periodontally diseased individuals,” Journal of Periodontology, vol. 73, no. 5, pp. 551–554, 2002. View at Publisher · View at Google Scholar · View at Scopus
  51. R. Diab-Ladki, B. Pellat, and R. Chahine, “Decrease in the total antioxidant activity of saliva in patients with periodontal diseases,” Clinical Oral Investigations, vol. 7, no. 2, pp. 103–107, 2003. View at Publisher · View at Google Scholar · View at Scopus
  52. M. Takane, N. Sugano, T. Ezawa, T. Uchiyama, and K. Ito, “A marker of oxidative stress in saliva: association with periodontally-involved teeth of a hopeless prognosis,” Journal of Oral Science, vol. 47, no. 1, pp. 53–57, 2005. View at Publisher · View at Google Scholar · View at Scopus
  53. D.-X. Tan, L. C. Manchester, R. J. Reiter, W.-B. Qi, M. Karbownik, and J. R. Calvo, “Significance of melatonin in antioxidative defense system: reactions and products,” Biological Signals and Receptors, vol. 9, no. 3-4, pp. 137–159, 2000. View at Publisher · View at Google Scholar · View at Scopus
  54. A. Cutando, G. Gómez-Moreno, J. Villalba, M. J. Ferrera, G. Escames, and D. Acuña-Castroviejo, “Relationship between salivary melatonin levels and periodontal status in diabetic patients,” Journal of Pineal Research, vol. 35, no. 4, pp. 239–244, 2003. View at Publisher · View at Google Scholar · View at Scopus
  55. C. C. Tsai, H. S. Chen, S. L. Chen et al., “Lipid peroxidation: a possible role in the induction and progression of chronic periodontitis,” Journal of Periodontal Research, vol. 40, no. 5, pp. 378–386, 2005. View at Publisher · View at Google Scholar · View at Scopus
  56. Y. Karincaoglu, K. Batcioglu, T. Erdem, M. Esrefoglu, and M. Gene, “The levels of plasma and salivary antioxidants in the patient with recurrent aphthous stomatitis,” Journal of Oral Pathology and Medicine, vol. 34, no. 1, pp. 7–12, 2005. View at Publisher · View at Google Scholar · View at Scopus
  57. C. S. Miller, J. D. Foley, A. L. Bailey et al., “Current developments in salivary diagnostics,” Biomarkers in Medicine, vol. 4, no. 1, pp. 171–189, 2010. View at Publisher · View at Google Scholar · View at Scopus
  58. X. Gao, S. Jiang, D. Koh, and C. S. Hsu, “Salivary biomarkers for dental caries,” Periodontology 2000, vol. 70, no. 1, pp. 128–141, 2016. View at Publisher · View at Google Scholar
  59. Y. Hassona and C. Scully, “Salivary changes in oral mucosal diseases,” Periodontology 2000, vol. 70, no. 1, pp. 111–127, 2016. View at Publisher · View at Google Scholar
  60. A. M. Schmidt, E. Weidman, E. Lalla et al., “Advanced glycation endproducts (AGEs) induce oxidant stress in the gingiva: a potential mechanism underlying accelerated periodontal disease associated with diabetes,” Journal of Periodontal Research, vol. 31, no. 7, pp. 508–515, 1996. View at Publisher · View at Google Scholar · View at Scopus
  61. S. A. Morenkova, “Comparative analysis of dependence of saliva sorbitol and fructoseamine levels on blood glucose level in patients with diabetes,” Biomeditsinskaya Khimiya, vol. 50, no. 6, pp. 612–614, 2004 (Russian). View at Google Scholar · View at Scopus
  62. F. Astaneie, M. Afshari, A. Mojtahedi et al., “Total antioxidant capacity and levels of epidermal growth factor and nitric oxide in blood and saliva of insulin-dependent diabetic patients,” Archives of Medical Research, vol. 36, no. 4, pp. 376–381, 2005. View at Publisher · View at Google Scholar · View at Scopus
  63. M. E. Lopez, M. E. Colloca, R. G. Páez, J. N. Schallmach, M. A. Koss, and A. Chervonagura, “Salivary characteristics of diabetic children,” Brazilian Dental Journal, vol. 14, no. 1, pp. 26–31, 2003. View at Publisher · View at Google Scholar · View at Scopus
  64. R. Wolfram, A. Oguogho, B. Palumbo, and H. Sinzinger, “Enhanced oxidative stress in coronary heart disease and chronic heart failure as indicated by an increased 8-epi-PGF2α,” European Journal of Heart Failure, vol. 7, no. 2, pp. 167–172, 2005. View at Publisher · View at Google Scholar · View at Scopus
  65. S. Xiang, R. Denver, M. Bailey, and H. Krum, “Physiologic determinants of endothelin concentrations in human saliva,” Clinical Chemistry, vol. 49, no. 12, pp. 2012–2019, 2003. View at Publisher · View at Google Scholar · View at Scopus
  66. N. Christodoulides, F. N. Pierre, X. Sanchez et al., “Programmable bio-nanochip technology for the diagnosis of cardiovascular disease at the point-of-care,” Methodist DeBakey Cardiovascular Journal, vol. 8, no. 1, pp. 6–12, 2012. View at Publisher · View at Google Scholar · View at Scopus
  67. E. J. Franzmann, E. P. Reategui, K. L. Carraway, K. L. Hamilton, D. T. Weed, and W. J. Goodwin, “Salivary soluble CD44: a potential molecular marker for head and neck cancer,” Cancer Epidemiology Biomarkers and Prevention, vol. 14, no. 3, pp. 735–739, 2005. View at Publisher · View at Google Scholar · View at Scopus
  68. L.-P. Zhong, G.-F. Chen, Z.-F. Xu, X. Zhang, F.-Y. Ping, and S.-F. Zhao, “Detection of telomerase activity in saliva from oral squamous cell carcinoma patients,” International Journal of Oral and Maxillofacial Surgery, vol. 34, no. 5, pp. 566–570, 2005. View at Publisher · View at Google Scholar · View at Scopus
  69. A. K. Markopoulos, E. Z. Michailidou, and G. Tzimagiorgis, “Salivary markers for oral cancer detection,” Open Dentistry Journal, no. 4, pp. 172–178, 2010. View at Google Scholar
  70. F. D. Shah, R. Begum, B. N. Vajaria et al., “A review on salivary genomics and proteomics biomarkers in oral cancer,” Indian Journal of Clinical Biochemistry, vol. 26, no. 4, pp. 326–334, 2011. View at Publisher · View at Google Scholar · View at Scopus
  71. C. F. Streckfus, L. Bigler, T. Dellinger, M. Kuhn, N. Chouinard, and X. Dai, “The expression of the c-erbB-2 receptor protein in glandular salivary secretions,” Journal of Oral Pathology and Medicine, vol. 33, no. 10, pp. 595–600, 2004. View at Publisher · View at Google Scholar · View at Scopus
  72. M. Markman, “Normal CA-125 and the risk of progression in ovarian cancer,” Journal of Clinical Oncology, vol. 24, no. 20, p. 3310, 2006. View at Publisher · View at Google Scholar · View at Scopus
  73. L. Zhang, J. J. Farrell, H. Zhou et al., “Salivary transcriptomic biomarkers for detection of resectable pancreatic cancer,” Gastroenterology, vol. 138, no. 3, pp. 949–957, 2010. View at Publisher · View at Google Scholar · View at Scopus
  74. C. T. Viet and B. L. Schmidt, “Methylation array analysis of preoperative and postoperative saliva DNA in oral cancer patients,” Cancer Epidemiology, Biomarkers and Prevention, vol. 17, no. 12, pp. 3603–3611, 2008. View at Publisher · View at Google Scholar · View at Scopus
  75. J. Wei, G. Xie, Z. Zhou et al., “Salivary metabolite signatures of oral cancer and leukoplakia,” International Journal of Cancer, vol. 129, no. 9, pp. 2207–2217, 2011. View at Publisher · View at Google Scholar · View at Scopus
  76. D. L. Mager, A. D. Haffajee, P. M. Delvin, C. M. Norris, M. R. Posner, and J. M. Goodson, “The salivary microbiota as a diagnostic indicator of oral cancer: a descriptive, non-randomized study of cancer-free and oral squamous cell carcinoma subjects,” Journal of Translational Medicine, vol. 3, article 27, 2005. View at Publisher · View at Google Scholar · View at Scopus
  77. H. Xiao, L. Zhang, H. Zhou, J. M. Lee, E. B. Garon, and D. T. W. Wong, “Proteomic analysis of human saliva from lung cancer patients using two-dimensional difference gel electrophoresis and mass spectrometry,” Molecular & Cellular Proteomics, vol. 11, no. 2, 2012. View at Publisher · View at Google Scholar · View at Scopus
  78. P. S. Ramsey and W. W. Andrews, “Biochemical predictors of preterm labor: fetal fibronectin and salivary estriol,” Clinics in Perinatology, vol. 30, no. 4, pp. 701–733, 2003. View at Publisher · View at Google Scholar · View at Scopus
  79. L. D. Belkien, J. Bordt, P. Moller, R. Hano, and E. Nieschlag, “Estradiol in saliva for monitoring follicular stimulation in an in vitro fertilization program,” Fertility and Sterility, vol. 44, no. 3, pp. 322–327, 1985. View at Google Scholar · View at Scopus
  80. K. Simunkova, R. Hampl, M. Hill, J. Doucha, L. Starka, and K. Vondra, “Salivary cortisol in low dose (1 microg) ACTH test in healthy women: comparison with serum cortisol,” Physiological Research, vol. 56, no. 4, pp. 449–453, 2007. View at Google Scholar
  81. A. van Stegeren, N. Rohleder, W. Everaerd, and O. T. Wolf, “Salivary alpha amylase as marker for adrenergic activity during stress: effect of betablockade,” Psychoneuroendocrinology, vol. 31, no. 1, pp. 137–141, 2006. View at Publisher · View at Google Scholar · View at Scopus
  82. R. S. Rittmaster and D. L. Thompson, “Effect of leuprolide and dexamethasone on hair growth and hormone levels in hirsute women: the relative importance of the ovary and the adrenal in the pathogenesis of hirsutism,” Journal of Clinical Endocrinology and Metabolism, vol. 70, no. 4, pp. 1096–1102, 1990. View at Publisher · View at Google Scholar · View at Scopus
  83. I. M. Goodyer, J. Herbert, and A. Tamplin, “Psychoendocrine antecedents of persistent first-episode major depression in adolescents: a community-based longitudinal enquiry,” Psychological Medicine, vol. 33, no. 4, pp. 601–610, 2003. View at Publisher · View at Google Scholar · View at Scopus
  84. S. Yamada, “Assessment of mental condition by saliva level of 3-methoxy-4-hydroxyphenylglycol,” Seishin Shinkeigaku Zasshi, vol. 107, no. 7, pp. 712–717, 2005 (Japanese). View at Google Scholar · View at Scopus
  85. M. Tsuruoka, J. Hara, A. Hirayama et al., “Capillary electrophoresis-mass spectrometry-based metabolome analysis of serum and saliva from neurodegenerative dementia patients,” Electrophoresis, vol. 34, no. 19, pp. 2865–2872, 2013. View at Publisher · View at Google Scholar · View at Scopus
  86. N. Pant Pai, B. Balram, S. Shivkumar et al., “Head-to-head comparison of accuracy of a rapid point-of-care HIV test with oral versus whole-blood specimens: a systematic review and meta-analysis,” The Lancet Infectious Diseases, vol. 12, no. 5, pp. 373–380, 2012. View at Publisher · View at Google Scholar · View at Scopus
  87. P. L. Corstjens, W. R. Abrams, and D. Malamud, “Saliva and viral infections,” Periodontology 2000, vol. 70, no. 1, pp. 93–110, 2016. View at Publisher · View at Google Scholar
  88. G. B. L. Menezes, F. A. Pereira, C. A. B. Duarte et al., “Hepatitis C virus quantification in serum and saliva of HCV-infected patients,” Memorias do Instituto Oswaldo Cruz, vol. 107, no. 5, pp. 680–683, 2012. View at Publisher · View at Google Scholar · View at Scopus
  89. G. Yap, B. K. Sil, and L.-C. Ng, “Use of saliva for early dengue diagnosis,” PLoS Neglected Tropical Diseases, vol. 5, no. 5, Article ID e1046, 2011. View at Publisher · View at Google Scholar · View at Scopus
  90. P. L. A. M. Corstjens and D. Malamud, “Point-of-care diagnostics for infectious diseases,” in Salivary Diagnostics, D. T. Wong, Ed., chapter 13, pp. 136–149, Wiley-Blackwell, Ames, Iowa, USA, 2008. View at Google Scholar
  91. J. W. Galloway, B. J. F. Keijser, and D. M. Williams, “Saliva in studies of epidemiology of human disease: the UK Biobank project,” Periodontology 2000, vol. 70, no. 1, pp. 184–195, 2016. View at Publisher · View at Google Scholar