Heat Shock Protein HSP27 Secretion by Ovarian Cancer Cells Is Linked to Intracellular Expression Levels, Occurs Independently of the Endoplasmic Reticulum Pathway and HSP27’s Phosphorylation Status, and Is Mediated by Exosome Liberation

Figure 6

Load of HSP27 in exosomes from heat shock-treated SK-OV-3 cells. (a) SK-OV-3 cells were treated with a 37°C/39°C/37°C heat shock cycle for 24 h each temperature (heat shock) and compared to cells incubated by 37°C for 72 h (control). Relative intracellular HSP27 expression in cell lysates and relative HSP27 load in prepared exosomes was determined by Western blotting. HSP27 signal was normalized to GAPDH. (b) Representative Western blots of experiments as described in Figure 3(a). P values were determined by Student’s -test with () and () given as significant.

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